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Master thesis

Cartographie de la voie médiée par les récepteurs aux neurotrophines dans la région Aorte Gonade Mésonéphros chez l’embryon de souris

Abstract : The first hematopoietic stem cells (HSCs) emerge in the Aorta-Gonad-Mesonephros (AGM) region in the vertebrate embryo. This process takes place during a short developmental period, between days 10.5 and 12.5 in the mouse embryo. More specifically, HSCs arise from so-called hemogenic aortic endothelial cells in the ventral region of the dorsal aorta. In order to better characterize the hematopoietic microenvironment of the AGM, we explored the molecular signature of the tissues surrounding the dorsal aorta by combining approaches of laser microdissection, DNA chips and bioinformatics analyses. This study showed that the Ntrk2 and Ntrk3 genes, encoding two neurotrophin receptors (the TRKB and TRKC proteins), are preferentially expressed in the cells underlying the dorsal aorta. The main objective of my project is to comprehensively map the neurotrophin-mediated pathway in AGM using single-cell molecular biology, imaging and transcriptomic approaches. My results suggest that 1) TRKB and TRKC receptor expression is dynamic along the anteroposterior axis of the mouse embryo, 2) TRKB+ and TRKC+ populations exhibit phenotypic and molecular differences, and 3) the Expression of genes encoding neurotrophins (Bdnf, Ntf3 and Ntf5) is heterogeneous and associated with neural (Bdnf), mesenchymal (Bdnf, Ntf3 and Ntf5) and smooth muscle (Bdnf) cells. My results also support the existence of a population of neuro-mesenchymal cells in the subaortic region. Functional approaches are now being considered to study the functional role of the neurotrophin-mediated pathway in aortic hematopoiesis.
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Master thesis
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https://hal-ephe.archives-ouvertes.fr/hal-03545712
Contributor : Pierre-Yves Canto Connect in order to contact the contributor
Submitted on : Thursday, January 27, 2022 - 2:01:43 PM
Last modification on : Saturday, February 12, 2022 - 3:46:18 AM

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  • HAL Id : hal-03545712, version 1

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Pierre-Yves Canto. Cartographie de la voie médiée par les récepteurs aux neurotrophines dans la région Aorte Gonade Mésonéphros chez l’embryon de souris. Sciences du Vivant [q-bio]. 2021. ⟨hal-03545712⟩

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