B. M. Abell, M. Hahn, L. A. Holbrook, and M. M. Moloney, Membrane topology and sequence requirements for oil body targeting of oleosin, Plant J, vol.37, pp.461-470, 2004.

A. J. Bel, W. J. Kesteren, and . Van, Plasmodesmata: Structure, Function, Role in Cell Communication, 2012.

G. Bertani, STUDIES ON LYSOGENESIS I, J. Bacteriol, vol.62, pp.293-300, 1951.

S. Bloemendal and U. Kück, Cell-to-cell communication in plants, animals, and fungi: a comparative review, Naturwissenschaften, vol.100, pp.3-19, 2013.

L. C. Boavida and S. Mccormick, TECHNICAL ADVANCE: Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana, Plant J, vol.52, pp.570-582, 2007.

P. Boevink, K. Oparka, S. Santa-cruz, B. Martin, A. Betteridge et al., Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network, Plant J. Cell Mol. Biol, vol.15, pp.441-447, 1998.

N. A. Boggs, J. B. Nasrallah, and M. E. Nasrallah, Independent S-Locus Mutations Caused Self-Fertility in Arabidopsis thaliana, PLOS Genet, vol.5, 2009.

S. Bolte, C. Talbot, Y. Boutte, O. Catrice, N. D. Read et al., FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells, J. Microsc, vol.214, pp.159-173, 2004.
URL : https://hal.archives-ouvertes.fr/hal-00122335

J. L. Bowman, D. R. Smyth, and E. M. Meyerowitz, Genetic interactions among floral homeotic genes of Arabidopsis, Development, vol.112, pp.1-20, 1991.

D. Cabrillac, J. M. Cock, C. Dumas, and T. Gaude, The S -locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins, Nature, vol.410, p.220, 2001.
URL : https://hal.archives-ouvertes.fr/hal-01925586

C. Cameron and A. Geitmann, Cell mechanics of pollen tube growth, Curr. Opin. Genet. Dev, vol.51, pp.11-17, 2018.

C. Cameron and A. Geitmann, Cell mechanics of pollen tube growth, Curr. Opin. Genet. Dev, vol.51, pp.11-17, 2018.

A. L. Carter and T. Mcneilly, Increased atmospheric humidity post pollination: A possible aid to the production of inbred line seed from mature flowers in the Brussels sprout (Brassica oleracea var. Gemmifera), Euphytica, vol.25, pp.531-538, 1976.

J. Chaiwanon, W. Wang, J. Zhu, E. Oh, and Z. Wang, Information Integration and Communication in Plant Growth Regulation, Cell, vol.164, pp.1257-1268, 2016.

L. A. Chapman and D. R. Goring, Pollen-pistil interactions regulating successful fertilization in the Brassicaceae, J. Exp. Bot, vol.61, 1987.

W. Chen, X. Yin, L. Wang, J. Tian, R. Yang et al., Involvement of rose aquaporin RhPIP1;1 in ethylene-regulated petal expansion through interaction with RhPIP2, Plant Mol. Biol, vol.1, pp.219-233, 2013.

A. Y. Cheung, Q. Duan, S. S. Costa, B. H. De-graaf, V. S. Di-stilio et al., The Dynamic Pollen Tube Cytoskeleton: Live Cell Studies Using Actin-Binding and Microtubule-Binding Reporter Proteins, Mol. Plant, vol.1, pp.686-702, 2008.

M. L. Cilia, J. , and D. , Plasmodesmata form and function, Curr. Opin. Cell Biol, vol.16, pp.500-506, 2004.

S. E. Clark, M. P. Running, and E. M. Meyerowitz, CLAVATA1, a regulator of meristem and flower development in Arabidopsis, Development, vol.119, pp.397-418, 1993.

S. J. Clough and A. F. Bent, Floral dip: a simplified method forAgrobacteriummediated transformation ofArabidopsis thaliana, Plant J, vol.16, pp.735-743, 1998.

H. Cochard, J. Venisse, T. S. Barigah, N. Brunel, S. Herbette et al., Putative Role of Aquaporins in Variable Hydraulic Conductance of Leaves in Response to Light, Plant Physiol, vol.143, pp.122-133, 2007.
URL : https://hal.archives-ouvertes.fr/hal-01189217

N. Courtemanche, T. D. Pollard, C. , and Q. , Avoiding artifacts when counting polymerized actin in live cells with Lifeact-fluorescent fusion proteins, Nat. Cell Biol, vol.18, pp.676-683, 2016.

M. Cresti, S. Blackmore, J. L. Went, and . Van, Atlas of Sexual Reproduction in Flowering Plants, 1992.

H. Dickinson, Dry stigmas, water and self-incompatibility in Brassica, Sex. Plant Reprod, vol.8, pp.1-10, 1995.

H. G. Dickinson and C. J. Elleman, Structural changes in the pollen grain of Brassica oleracea during dehydration in the anther and development on the stigma as revealed by anhydrous fixation techniques, Micron Microsc. Acta, vol.16, pp.255-270, 1985.

B. Ding, A. Itaya, and Y. Woo, Plasmodesmata and Cell-to-Cell Communication in Plants, International Review of, pp.251-316, 1999.

J. Doughty, F. Hedderson, A. Mccubbin, and H. Dickinson, Interaction between a coating-borne peptide of the Brassica pollen grain and stigmatic S (selfincompatibility)-locus-specific glycoproteins, Proc. Natl. Acad. Sci. U. S. A, vol.90, p.467, 1993.

J. Doughty, S. Dixon, S. J. Hiscock, A. C. Willis, I. A. Parkin et al., PCP-A1, a Defensin-like Brassica Pollen Coat Protein That Binds the S Locus Glycoprotein, Is the Product of Gametophytic Gene Expression, Plant Cell, vol.10, pp.1333-1347, 1998.

J. Doughty, H. Y. Wong, D. , and H. G. , Cysteine-rich Pollen Coat Proteins (PCPs) and their Interactions with Stigmatic S (Incompatibility) and S-Related Proteins in Brassica: Putative Roles in SI and Pollination, Ann. Bot, vol.85, pp.161-169, 2000.

A. F. Edlund, R. Swanson, and D. Preuss, Pollen and Stigma Structure and Function: The Role of Diversity in Pollination, Plant Cell, vol.16, pp.84-97, 2004.

A. F. Edlund, R. Swanson, and D. Preuss, Pollen and stigma structure and function: the role of diversity in pollination, Plant Cell, vol.16, pp.84-97, 2004.

A. F. Edlund, Q. Zheng, N. Lowe, S. Kuseryk, K. L. Ainsworth et al., Pollen from Arabidopsis thaliana and other Brassicaceae are functionally omniaperturate, Am. J. Bot, vol.103, pp.1006-1019, 2016.

C. J. Elleman and H. G. Dickinson, The role of the exine coating in pollenstigma interactions in Brassica oleracea L, New Phytol, vol.114, pp.511-518, 1990.

C. J. Elleman and H. G. Dickinson, Identification of pollen components regulating pollination-specific responses in the stigmatic papillae of Brassica oleracea, New Phytol, vol.133, pp.197-205, 1996.

C. J. Elleman, V. Franklin-tong, and H. G. Dickinson, Pollination in species with dry stigmas: the nature of the early stigmatic response and the pathway taken by pollen tubes, New Phytol, vol.121, pp.413-424, 1992.

X. Feng and H. G. Dickinson, Tapetal cell fate, lineage and proliferation in the Arabidopsis anther, Development, vol.137, pp.2409-2416, 2010.

Y. Fu, G. Wu, Y. , and Z. , ROP Gtpase-Dependent Dynamics of Tip-Localized F-Actin Controls Tip Growth in Pollen Tubes, J. Cell Biol, vol.152, pp.1019-1032, 2001.

N. Geldner, V. Dénervaud-tendon, D. L. Hyman, U. Mayer, Y. Stierhof et al., Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set, Plant J. Cell Mol. Biol, vol.59, pp.69-78, 2009.

R. M. Hackett, G. Cadwallader, and F. C. Franklin, Functional analysis of a Brassica oleracea SLR1 gene promoter, Plant Physiol, vol.112, pp.1601-1607, 1996.

K. Haga and T. Sakai, PIN Auxin Efflux Carriers Are Necessary for Pulse-Induced But Not Continuous Light-Induced Phototropism in Arabidopsis, Plant Physiol, vol.160, pp.763-776, 2012.

O. Hazak and C. S. Hardtke, CLAVATA 1-type receptors in plant development, J. Exp. Bot, vol.67, pp.4827-4833, 2016.

Y. He, J. Zhou, L. Shan, and X. Meng, Plant cell surface receptor-mediated signaling -a common theme amid diversity, J Cell Sci, vol.131, 2018.

P. K. Hepler, C. M. Rounds, and L. J. Winship, Control of Cell Wall Extensibility during Pollen Tube Growth, Mol. Plant, vol.6, pp.998-1017, 2013.

J. Heslop-harrison, An Interpretation of the Hydrodynamics of Pollen, Am. J. Bot, vol.66, pp.737-743, 1979.

Y. Heslop-harrison and K. R. Shivanna, The Receptive Surface of the Angiosperm Stigma, Ann. Bot, vol.41, pp.1233-1258, 1977.

K. Hiroi, M. Sone, S. Sakazono, M. Osaka, H. Masuko-suzuki et al., Time-lapse imaging of self-and crosspollinations in Brassica rapa, Ann. Bot, vol.112, pp.115-122, 2013.

S. J. Hiscock, A. , and A. M. , Diverse cell signalling pathways regulate pollenstigma interactions: the search for consensus, New Phytol, vol.179, pp.286-317, 2008.

S. Huang, Y. Q. An, J. M. Mcdowell, E. C. Mckinney, and R. B. Meagher, The Arabidopsis ACT11 actin gene is strongly expressed in tissues of the emerging inflorescence, pollen, and developing ovules, Plant Mol. Biol, vol.33, pp.125-139, 1997.

B. Igic, R. Lande, and J. R. Kohn, Loss of Self-Incompatibility and Its Evolutionary Consequences, Int. J. Plant Sci, vol.169, pp.93-104, 2008.

A. Itaya, Y. Woo, C. Masuta, Y. Bao, R. S. Nelson et al., , 1998.

, Developmental Regulation of Intercellular Protein Trafficking through Plasmodesmata in Tobacco Leaf Epidermis, Plant Physiol, vol.118, pp.373-385

M. Iwano, H. Shiba, T. Miwa, F. Che, S. Takayama et al., Ca2+ Dynamics in a Pollen Grain and Papilla Cell during Pollination of Arabidopsis, Plant Physiol, vol.136, pp.3562-3571, 2004.

M. Iwano, H. Shiba, K. Matoba, T. Miwa, M. Funato et al., Actin dynamics in papilla cells of Brassica rapa during self-and cross-pollination, Plant Physiol, vol.144, pp.72-81, 2007.

M. Iwano, M. Igarashi, Y. Tarutani, P. Kaothien-nakayama, H. Nakayama et al., A Pollen Coat-Inducible Autoinhibited Ca2+-ATPase Expressed in Stigmatic Papilla Cells Is Required for Compatible Pollination in the Brassicaceae, 2014.

, Plant Cell, vol.26, pp.636-649

M. Iwano, K. Ito, S. Fujii, M. Kakita, H. Asano-shimosato et al., Calcium signalling mediates self-incompatibility response in the Brassicaceae, Nat. Plants, vol.1, p.15128, 2015.

M. Iwano, K. Ito, S. Fujii, M. Kakita, H. Asano-shimosato et al., Calcium signalling mediates self-incompatibility response in the Brassicaceae, Nat. Plants, vol.1, p.15128, 2015.

S. Jeong, A. E. Trotochaud, and S. E. Clark, The Arabidopsis CLAVATA2 gene encodes a receptor-like protein required for the stability of the CLAVATA1 receptorlike kinase, Plant Cell, vol.11, pp.1925-1934, 1999.

A. Kachroo, M. E. Nasrallah, and J. B. Nasrallah, Self-Incompatibility in the Brassicaceae, Plant Cell, vol.14, pp.227-238, 2002.

M. K. Kandasamy, J. B. Nasrallah, and M. E. Nasrallah, Pollen-pistil interactions and developmental regulation of pollen tube growth in Arabidopsis, Development, vol.120, pp.3405-3418, 1994.

H. Kitashiba and J. B. Nasrallah, Self-incompatibility in Brassicaceae crops: lessons for interspecific incompatibility, Breed. Sci, vol.64, pp.23-37, 2014.

H. Kitashiba, P. Liu, T. Nishio, J. B. Nasrallah, and M. E. Nasrallah, Functional test of Brassica self-incompatibility modifiers in Arabidopsis thaliana, Proc. Natl. Acad. Sci. U. S. A, vol.108, pp.18173-18178, 2011.

B. D. Kohorn, S. Johansen, A. Shishido, T. Todorova, R. Martinez et al., Pectin activation of MAP kinase and gene expression is WAK2 dependent, Plant J. Cell Mol. Biol, vol.60, pp.974-982, 2009.

M. Kusaba, K. Dwyer, J. Hendershot, J. Vrebalov, J. B. Nasrallah et al., Self-Incompatibility in the Genus Arabidopsis: Characterization of the S Locus in the Outcrossing A. lyrata and Its Autogamous Relative A. thaliana, Plant Cell, vol.13, pp.627-643, 2001.

J. Lee, S. K. Cho, and R. Sager, Plasmodesmata and Noncell Autonomous Signaling in Plants, The Plant Plasma Membrane, pp.87-107, 2011.

K. A. Lennon, S. Roy, P. K. Hepler, and E. M. Lord, The structure of the transmitting tissue of Arabidopsis thaliana (L.) and the path of pollen tube growth, Sex. Plant Reprod, vol.11, pp.49-59, 1998.

M. Lévesque-lemay, D. Chabot, K. Hubbard, J. K. Chan, S. Miller et al., Tapetal oleosins play an essential role in tapetosome formation and protein relocation to the pollen coat, New Phytol, vol.209, pp.691-704, 2016.

M. Lévesque-lemay, D. Chabot, K. Hubbard, J. K. Chan, S. Miller et al., Tapetal oleosins play an essential role in tapetosome formation and protein relocation to the pollen coat, New Phytol, vol.209, pp.691-704, 2016.

X. Liu, C. Castro, Y. Wang, J. Noble, N. Ponvert et al., The Role of LORELEI in Pollen Tube Reception at the Interface of the Synergid Cell and Pollen Tube Requires the Modified Eight-Cysteine Motif and the Receptor-Like Kinase FERONIA, Plant Cell, vol.28, pp.1035-1052, 2016.

D. T. Luu, P. Heizmann, and C. Dumas, Pollen-Stigma Adhesion in Kale Is Not Dependent on the Self-(In)Compatibility Genotype, Plant Physiol, vol.115, pp.1221-1230, 1997.

J. Ma, Z. Liu, C. Chu, Z. Hu, X. Wang et al., Different regulatory processes control pollen hydration and germination in Arabidopsis, Sex. Plant Reprod, vol.25, pp.77-82, 2012.

N. Ma, J. Xue, Y. Li, X. Liu, F. Dai et al., Rh-PIP2;1, a Rose Aquaporin Gene, Is Involved in Ethylene-Regulated Petal Expansion, Plant Physiol, vol.148, pp.894-907, 2008.

A. Mamode-cassim, P. Gouguet, J. Gronnier, N. Laurent, V. Germain et al., Plant lipids: Key players of plasma membrane organization and function, Prog. Lipid Res, vol.73, pp.1-27, 2019.
URL : https://hal.archives-ouvertes.fr/hal-02408567

N. Matsushima and H. Miyashita, Leucine-Rich Repeat (LRR) Domains Containing Intervening Motifs in Plants, Biomolecules, vol.2, pp.288-311, 2012.

C. Maurel, Y. Boursiac, D. Luu, V. Santoni, Z. Shahzad et al., , 2015.

, Aquaporins in Plants, Physiol. Rev, vol.95, pp.1321-1358

J. A. Mayfield and D. Preuss, Rapid initiation of Arabidopsis pollination requires the oleosin-domain protein GRP17, Nat. Cell Biol, vol.2, pp.128-130, 2000.

R. Müller, A. Bleckmann, and R. Simon, The Receptor Kinase CORYNE of Arabidopsis Transmits the Stem Cell-Limiting Signal CLAVATA3 Independently of CLAVATA1, Plant Cell, vol.20, pp.934-946, 2008.

D. J. Murphy, The extracellular pollen coat in members of the Brassicaceae: composition, biosynthesis, and functions in pollination, Protoplasma, vol.228, p.31, 2006.

E. Murphy, S. Smith, and I. D. Smet, Small Signaling Peptides in Arabidopsis Development: How Cells Communicate Over a Short Distance, Plant Cell, vol.24, pp.3198-3217, 2012.

J. B. Nasrallah, Chapter Sixteen -Self-incompatibility in the Brassicaceae: Regulation and mechanism of self-recognition, Current Topics in Developmental Biology, U. Grossniklaus, pp.435-452, 2019.

M. E. Nasrallah and D. H. Wallace, Immunogenetics of self-incompatibility in Brassica oleracea L, Heredity, vol.22, p.519, 1967.

M. E. Nasrallah, P. Liu, and J. B. Nasrallah, Generation of Self-Incompatible Arabidopsis thaliana by Transfer of Two S Locus Genes from A. lyrata, Science, vol.297, pp.247-249, 2002.

M. E. Nasrallah, P. Liu, S. Sherman-broyles, N. A. Boggs, and J. B. Nasrallah, Natural variation in expression of self-incompatibility in Arabidopsis thaliana: Implications for the evolution of selfing, Proc. Natl. Acad. Sci, vol.101, pp.16070-16074, 2004.

S. Nou, M. Watanabe, A. Isogai, and K. Hinata, Comparison of S-alleles and S-glycoproteins between two wild populations of Brassica campestris in Turkey and Japan, Sex. Plant Reprod, vol.6, pp.79-86, 1993.

D. J. Ockendon, Pollen Tube Growth and the Site of the Incompatibility Reaction in Brassica oleracea, New Phytol, vol.71, pp.519-522, 1972.

R. L. Overall and L. M. Blackman, A model of the macromolecular structure of plasmodesmata, Trends Plant Sci, vol.1, pp.307-311, 1996.

I. A. Paponov, W. D. Teale, M. Trebar, I. Blilou, and K. Palme, The PIN auxin efflux facilitators: evolutionary and functional perspectives, Trends Plant Sci, vol.10, pp.170-177, 2005.

K. Porter and B. Day, From filaments to function: The role of the plant actin cytoskeleton in pathogen perception, signaling and immunity, J. Integr. Plant Biol, vol.58, pp.299-311, 2016.

D. Preuss, B. Lemieux, G. Yen, and R. W. Davis, A conditional sterile mutation eliminates surface components from Arabidopsis pollen and disrupts cell signaling during fertilization, Genes Dev, vol.7, pp.974-985, 1993.

A. Rahman, A. Bannigan, W. Sulaman, P. Pechter, E. B. Blancaflor et al.,

, Auxin, actin and growth of the Arabidopsis thaliana primary root, Plant J, vol.50, pp.514-528

S. Reichelt, A. E. Knight, T. P. Hodge, F. Baluska, J. Samaj et al., Characterization of the unconventional myosin VIII in plant cells and its localization at the post-cytokinetic cell wall, Plant J, vol.19, pp.555-567, 1999.

A. Roberts and K. Oparka, Plasmodesmata and the control of symplastic transport, Plant Cell Environ, vol.26, pp.103-124, 2003.

I. N. Roberts, A. D. Stead, D. J. Ockendon, and H. G. Dickinson, Pollen stigma interactions in Brassica oleracea, Theor. Appl. Genet, vol.58, pp.241-246, 1980.

I. N. Roberts, G. Harrod, and H. G. Dickinson, Pollen-stigma interactions in Brassica oleracea. I. Ultrastructure and physiology of the stigmatic papillar cells, J. Cell Sci, vol.66, pp.241-253, 1984.

D. Safavian and D. R. Goring, Secretory Activity Is Rapidly Induced in Stigmatic Papillae by Compatible Pollen, but Inhibited for Self-Incompatible Pollen in the Brassicaceae, PLOS ONE, vol.8, p.84286, 2013.

D. Safavian, Y. Zayed, E. Indriolo, L. Chapman, A. Ahmed et al., , 2015.

, RNA Silencing of Exocyst Genes in the Stigma Impairs the Acceptance of Compatible Pollen in Arabidopsis1, Plant Physiol, vol.169, pp.2526-2538

R. Sager and J. Lee, Plasmodesmata in integrated cell signalling: insights from development and environmental signals and stresses, J. Exp. Bot, vol.65, pp.6337-6358, 2014.

M. A. Samuel, Y. T. Chong, K. E. Haasen, M. G. Aldea-brydges, S. L. Stone et al., Cellular Pathways Regulating Responses to Compatible and Self-Incompatible Pollen in Brassica and Arabidopsis Stigmas Intersect at Exo70A1, a Putative Component of the Exocyst Complex, Plant Cell, vol.21, pp.2655-2671, 2009.

M. A. Samuel, W. Tang, M. Jamshed, J. Northey, D. Patel et al., Proteomic Analysis of Brassica Stigmatic Proteins Following the Self-incompatibility Reaction Reveals a Role for Microtubule Dynamics During Pollen Responses, Mol. Cell. Proteomics, vol.10, 2011.

A. M. Sanchez, M. Bosch, M. Bots, J. Nieuwland, R. Feron et al., , 2004.

, Pistil Factors Controlling Pollination, Plant Cell, vol.16, pp.98-106

M. R. Sánchez-villagra and I. Werneburg, Mammalian organogenesis in deep time: tools for teaching and outreach, Evol. Educ. Outreach, vol.9, p.11, 2016.

R. H. Sarker, C. J. Elleman, and H. G. Dickinson, Control of pollen hydration in Brassica requires continued protein synthesis, and glycosylation in necessary for intraspecific incompatibility, Proc. Natl. Acad. Sci. U. S. A, vol.85, pp.4340-4344, 1988.

C. R. Schopfer, M. E. Nasrallah, and J. B. Nasrallah, The Male Determinant of Self-Incompatibility in Brassica, Science, vol.286, pp.1697-1700, 1999.

C. R. Schopfer, M. E. Nasrallah, and J. B. Nasrallah, The Male Determinant of Self-Incompatibility in Brassica, Science, vol.286, pp.1697-1700, 1999.

S. Shiu and A. B. Bleecker, Plant Receptor-Like Kinase Gene Family: Diversity, Function, and Signaling, Sci STKE, pp.22-22, 2001.

D. R. Smyth, J. L. Bowman, and E. M. Meyerowitz, Early flower development in Arabidopsis, Plant Cell, vol.2, pp.755-767, 1990.

J. C. Stein and J. B. Nasrallah, A plant receptor-like gene, the S-locus receptor kinase of Brassica oleracea L., encodes a functional serine/threonine kinase, Plant Physiol, vol.101, pp.1103-1106, 1993.

J. C. Stein, B. Howlett, D. C. Boyes, M. E. Nasrallah, and J. B. Nasrallah, Molecular cloning of a putative receptor protein kinase gene encoded at the selfincompatibility locus of Brassica oleracea, Proc. Natl. Acad. Sci. U. S. A, vol.88, pp.8816-8820, 1991.

S. L. Stone, M. Arnoldo, and D. R. Goring, A Breakdown of Brassica Self-Incompatibility in ARC1 Antisense Transgenic Plants, Science, vol.286, pp.1729-1731, 1999.

S. L. Stone, E. M. Anderson, R. T. Mullen, and D. R. Goring, ARC1 Is an E3, 2003.

, Ubiquitin Ligase and Promotes the Ubiquitination of Proteins during the Rejection of Self-Incompatible Brassica Pollen, Plant Cell, vol.15, pp.885-898

R. Swanson, A. F. Edlund, and D. Preuss, Species Specificity in Pollen-Pistil Interactions, Annu. Rev. Genet, vol.38, pp.793-818, 2004.

S. Takayama, H. Shiba, M. Iwano, K. Asano, M. Hara et al., Isolation and characterization of pollen coat proteins of Brassica campestris that interact with S locus-related glycoprotein 1 involved in pollen-stigma adhesion, Proc. Natl. Acad. Sci, vol.97, pp.3765-3770, 2000.

S. Takayama, H. Shiba, M. Iwano, H. Shimosato, F. Che et al., The pollen determinant of selfincompatibility in Brassica campestris, Proc. Natl. Acad. Sci, vol.97, pp.1920-1925, 2000.

M. A. Titus, Myosins. Curr. Opin. Cell Biol, vol.5, pp.77-81, 1993.

A. E. Trotochaud, T. Hao, G. Wu, Z. Yang, and S. E. Clark, The CLAVATA1 receptor-like kinase requires CLAVATA3 for its assembly into a signaling complex that includes KAPP and a Rho-related protein, Plant Cell, vol.11, pp.393-406, 1999.

T. Tsuchimatsu, P. Kaiser, C. Yew, J. B. Bachelier, and K. K. Shimizu, Recent Loss of Self-Incompatibility by Degradation of the Male Component in Allotetraploid Arabidopsis kamchatica, PLOS Genet, vol.8, 2012.

D. Twell, J. Yamaguchi, and S. Mccormick, Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis, Development, vol.109, pp.705-713, 1990.

E. P. Updegraff, F. Zhao, and D. Preuss, The extracellular lipase EXL4 is required for efficient hydration of Arabidopsis pollen, Sex. Plant Reprod, vol.22, pp.197-204, 2009.

T. Vernoux, J. Kronenberger, O. Grandjean, P. Laufs, and J. Traas, PIN-FORMED 1 regulates cell fate at the periphery of the shoot apical meristem, Development, vol.127, pp.5157-5165, 2000.

G. Vervliet, M. Holsters, H. Teuchy, M. Van-montagu, and J. Schell, Characterization of Different Plaque-forming and Defective Temperate Phages in Agrobacterium Strains, J. Gen. Virol, vol.26, pp.33-48, 1975.

M. Watanabe, S. Takayama, A. Isogai, and K. Hinata, Recent Progresses on Self-incompatibility Research in Brassica Species, Breed. Sci. -Breed. SCI, vol.53, pp.199-208, 2003.

M. C. Webb and E. G. Williams, Effects of Temperature, Light, Nutrients and Carbon Dioxide on the Strength of the Self-Incompatibility Response in Detached Flowers of Lycopersicon peruvianum, Ann. Bot, vol.61, pp.395-404, 1988.

R. G. White, K. Badelt, R. L. Overall, and M. Vesk, Actin associated with plasmodesmata, Protoplasma, vol.180, pp.169-184, 1994.

S. Wolf, Plant cell wall signalling and receptor-like kinases, Biochem. J, vol.474, pp.471-492, 2017.

R. K. Yadav, M. Perales, J. Gruel, T. Girke, H. Jönsson et al., WUSCHEL protein movement mediates stem cell homeostasis in the Arabidopsis shoot apex, Genes Dev, vol.25, pp.2025-2030, 2011.

D. Yu, L. I. Na, L. I. Gang, L. I. Wen-long, Y. A. Agronomy et al., Actin and Myosin Co-Localize in Plasmodesmata and Ectodesmata-Like Structure, J. Integr. Agric, p.450002, 2011.

X. Zhang, R. Henriques, S. Lin, Q. Niu, and N. Chua, Agrobacteriummediated transformation of Arabidopsis thaliana using the floral dip method, Nat. Protoc, vol.1, p.641, 2006.

Y. Zheng, X. Lin, H. Liang, F. Wang, C. et al., The Long Journey of Pollen Tube in the Pistil, Int. J. Mol. Sci, vol.19, 2018.

G. M. Zinkl and D. Preuss, Dissecting Arabidopsis Pollen-Stigma Interactions Reveals Novel Mechanisms that Confer Mating Specificity, Ann. Bot, vol.85, pp.15-21, 2000.

G. M. Zinkl, B. I. Zwiebel, D. G. Grier, and D. Preuss, Pollen-stigma adhesion in Arabidopsis: a species-specific interaction mediated by lipophilic molecules in the pollen exine, Development, vol.126, pp.5431-5440, 1999.

M. I. Zuberi and H. G. Dickinson, Pollen-stigma interaction in Brassica. III. Hydration of the pollen grains, J. Cell Sci, vol.76, pp.321-336, 1985.

N. A. Boggs, K. G. Dwyer, M. E. Nasrallah, and J. B. Nasrallah, In Vivo Detection of Residues Required for Ligand-Selective Activation of the S-Locus Receptor in Arabidopsis, Current Biology, vol.19, pp.786-791, 2009.

D. Cabrillac, J. M. Cock, C. Dumas, and T. Gaude, The S -locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins, Nature, vol.410, pp.220-223, 2001.
URL : https://hal.archives-ouvertes.fr/hal-01925586

A. L. Carter and T. Mcneilly, Increased atmospheric humidity post pollination: A possible aid to the production of inbred line seed from mature flowers in the Brussels sprout (Brassica oleracea var. Gemmifera), Euphytica, vol.25, pp.531-538, 1976.

L. A. Chapman and D. R. Goring, Pollen-pistil interactions regulating successful fertilization in the Brassicaceae, Journal of Experimental Botany, vol.61, pp.1987-1999, 2010.

A. Y. Cheung, L. C. Boavida, M. Aggarwal, H. Wu, and J. A. Feijó, The pollen tube journey in the pistil and imaging the in vivo process by two-photon microscopy, Journal of Experimental Botany, vol.61, pp.1907-1915, 2010.

J. Dearnaley, K. M. Clark, I. B. Heath, R. R. Lew, and D. R. Goring, Neither compatible nor selfincompatible pollinations of Brassica napus involve reorganization of the papillar cytoskeleton, New phytologist, vol.141, pp.199-207, 1999.

H. G. Dickinson, Dry stigmas, water and self-incompatibility in Brassica, Sexual Plant Reproduction, vol.8, pp.1-10, 1995.

J. Doucet, H. K. Lee, and D. R. Goring, Pollen Acceptance or Rejection: A Tale of Two Pathways, Trends in Plant Science, vol.21, pp.1058-1067, 2016.

E. Durand, R. Meheust, and M. Soucaze, Dominance hierarchy arising from the evolution of a complex small RNA regulatory network, Science, vol.346, pp.1200-1205, 2014.
URL : https://hal.archives-ouvertes.fr/hal-01110837

A. Era, M. Tominaga, K. Ebine, C. Awai, C. Saito et al., Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha, Plant and Cell Physiology, vol.50, pp.1041-1048, 2009.

C. J. Elleman, H. Dickinson, E. G. Williams, R. B. Knox, and A. E. Clarke, Genetic Control of Self-Incompatibility and Reproductive Development in Flowering Plants, pp.67-87, 1994.

M. Grebe, J. Xu, W. Möbius, T. Ueda, A. Nakano et al., Arabidopsis sterol endocytosis involves actin-mediated trafficking via ARA6-positive early endosomes, Current Biology, vol.13, pp.1378-1387, 2003.

A. R. Hardham, D. Takemoto, and R. G. White, Rapid and dynamic subcellular reorganization following mechanical stimulation of Arabidopsis epidermal cells mimics responses to fungal and oomycete attack, BMC Plant Biology, vol.8, p.63, 2008.

K. Hiroi, M. Sone, S. Sakazono, M. Osaka, H. Masuko-suzuki et al., Time-lapse imaging of self-and cross-pollinations in Brassica rapa, Annals of Botany, vol.112, pp.115-122, 2013.

E. Indriolo, D. Safavian, and D. R. Goring, The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in Arabidopsis, The Plant Cell, vol.26, pp.1525-1543, 2014.

R. Ivanov, I. Fobis-loisy, and T. Gaude, When no means no: guide to Brassicaceae selfincompatibility, Trends in Plant Science, vol.15, pp.387-394, 2010.
URL : https://hal.archives-ouvertes.fr/hal-02353863

M. Iwano, H. Shiba, T. Miwa, F. S. Che, S. Takayama et al., Ca2+ Dynamics in a Pollen Grain and Papilla Cell during Pollination of Arabidopsis, Plant Physiology, vol.136, pp.3562-3571, 2004.

M. Iwano, H. Shiba, and K. Matoba, Actin Dynamics in Papilla Cells of Brassica rapa during Self-and Cross-Pollination, Plant Physiology, vol.144, pp.72-81, 2007.

Y. Jaillais, I. Fobis-loisy, C. Miège, and T. Gaude, Evidence for a sorting endosome in Arabidopsis root cells: Plant sorting endosome, The Plant Journal, vol.53, pp.237-247, 2007.

J. E. Nelles, H. Goring, and D. R. , The Molecular and Cellular Regulation of Brassicaceae Self-Incompatibility and Self-Pollen Rejection, International Review of Cell and Molecular Biology, pp.1-35, 2019.

A. Jelínková, K. Malínská, and S. Simon, Probing plant membranes with FM dyes: tracking, dragging or blocking?, The Plant Journal, vol.61, pp.883-892, 2010.

M. K. Kandasamy, J. B. Nasrallah, and M. E. Nasrallah, Pollen-pistil interactions and developmental regulation of pollen tube growth in Arabidopsis, Development, vol.120, pp.3405-3418, 1994.

Y. O. Kho and J. Baër, Observing pollen tubes by means of fluorescence, Euphytica, vol.17, pp.298-302, 1968.

H. Kitashiba, P. Liu, T. Nishio, J. B. Nasrallah, and M. E. Nasrallah, Functional test of Brassica selfincompatibility modifiers in Arabidopsis thaliana, Proceedings of the National Academy of Sciences, vol.108, pp.18173-18178, 2011.

B. A. Lalonde, M. E. Nasrallah, K. G. Dwyer, C. Chen, B. Barlow et al., A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene, The Plant Cell, vol.1, pp.249-258, 1989.

P. Liu, S. Sherman-broyles, M. E. Nasrallah, and J. B. Nasrallah, A Cryptic Modifier Causing Transient Self-Incompatibility in Arabidopsis thaliana, Current Biology, vol.17, pp.734-740, 2007.

M. E. Nasrallah and J. B. Nasrallah, Generation of Self-Incompatible Arabidopsis thaliana by Transfer of Two S Locus Genes from A. lyrata, Science, vol.297, pp.247-249, 2002.

M. E. Nasrallah, P. Liu, S. Sherman-broyles, N. A. Boggs, and J. B. Nasrallah, Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing, Proceedings of the National Academy of Sciences of the United States of America, vol.101, pp.16070-16074, 2004.

J. B. Nasrallah and M. E. Nasrallah, S -locus receptor kinase signalling, Biochemical Society Transactions, vol.42, pp.313-319, 2014.

D. Nettancourt and . De, Incompatibility and Incongruity in Wild and Cultivated Plants, 2001.

D. J. Ockendon, Effect of hexane and humidity on self-incompatibility in Brassica oleracea, Theoretical and Applied Genetics, vol.52, pp.113-117, 1978.

R. Palanivelu and D. Preuss, Distinct short-range ovule signals attract or repel Arabidopsis thaliana pollen tubes in vitro, BMC Plant Biology, vol.6, p.7, 2006.

D. Safavian and D. R. Goring, Secretory Activity Is Rapidly Induced in Stigmatic Papillae by Compatible Pollen, but Inhibited for Self-Incompatible Pollen in the Brassicaceae (D Bassham, PLoS ONE, vol.8, p.84286, 2013.

M. A. Samuel, Y. T. Chong, K. E. Haasen, M. G. Aldea-brydges, S. L. Stone et al., Cellular Pathways Regulating Responses to Compatible and Self-Incompatible Pollen in Brassica and Arabidopsis Stigmas Intersect at Exo70A1, a Putative Component of the Exocyst Complex, THE PLANT CELL ONLINE, vol.21, pp.2655-2671, 2009.

C. R. Schopfer, M. E. Nasrallah, and J. B. Nasrallah, The Male Determinant of Self-Incompatibility in Brassica, Science, vol.286, pp.1697-1700, 1999.

D. R. Smyth, J. L. Bowman, and E. M. Meyerowitz, Early flower development in Arabidopsis, The Plant Cell, vol.2, pp.755-767, 1990.

J. C. Stein, B. Howlett, D. C. Boyes, M. E. Nasrallah, and J. B. Nasrallah, Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea, Proceedings of the National Academy of Sciences, vol.88, pp.8816-8820, 1991.

D. Szymanski and C. J. Staiger, The Actin Cytoskeleton: Functional Arrays for Cytoplasmic Organization and Cell Shape Control, Plant Physiology, vol.176, pp.106-118, 2018.

S. Takayama, H. Shiba, M. Iwano, H. Shimosato, C. et al., The pollen determinant of self-incompatibility in Brassica campestris, Proceedings of the National Academy of Sciences, vol.97, pp.1920-1925, 2000.

D. Takemoto and A. R. Hardham, The Cytoskeleton as a Regulator and Target of Biotic Interactions in Plants, Plant Physiology, vol.136, pp.3864-3876, 2004.

C. W. Tung, K. G. Dwyer, M. E. Nasrallah, and J. B. Nasrallah, Genome-Wide Identification of Genes Expressed in Arabidopsis Pistils Specifically along the Path of Pollen Tube Growth, Plant Physiology, vol.138, pp.977-989, 2005.

F. Vogler, S. Konrad, and S. Sprunck, Knockin' on pollen's door: live cell imaging of early polarization events in germinating Arabidopsis pollen, Frontiers in Plant Science, vol.6, 2015.

L. Wang, L. A. Clarke, R. J. Eason, C. C. Parker, B. Qi et al., PCP-B class pollen coat proteins are key regulators of the hydration checkpoint in Arabidopsis thaliana pollen-stigma interactions, New Phytologist, 2016.

X. Yang, Q. Zhang, K. Zhao, Q. Luo, S. Bao et al., The Arabidopsis GPR1 Gene Negatively Affects Pollen Germination, Pollen Tube Growth, and Gametophyte Senescence, International Journal of Molecular Sciences, vol.18, 2017.

M. I. Zuberi and H. G. Dickinson, Pollen-stigma interaction in Brassica. III. Hydration of the pollen grains, Journal of Cell Science, vol.76, pp.321-336, 1985.

. Hackett, Karini et al. 2002) for expression of transgenes in A. thaliana. The DNA fragment containing the Brassica oleracea SLR1 stigma specific promoter (1.5 kb upstream of the SLR1 start codon, We used Gateway® vectors, 1996.

. Fobis-loisy,

. Durand, which were inserted by recombination into the plasmid pDONP4-P1R. Coding sequence (CDS) of TURQUOISE (TURQ), GFP or RFP proteins were introduced in the pDONP2R-P3 (+Stop), pp.4-5, 1989.

, The SLR1 promoter, the genomic sequence AlSRK14 and a 3'mock sequence were inserted in the pK7m34GW destination vectors. The genomic sequence AlSCR14 (including promoter and 3'UTR regions), a 5'mock sequence and a 3'mock sequence were inserted in the pB7m34GW. The SLR1 promoter, the LifeActin:Venus and a 3'mock sequence were inserted in the pB7m34GW (Act:Venus). The LAT52 promoter, the TURQUOISE CDS (+stop) and a 3'mock sequence were inserted in the pK7m34GW. The ACT11 promoter, the RFP CDS (+stop) and a 3'mock sequence were

, Arabidopsis transgenic plants were generated using Agrobacterium tumefaciens-mediated transformation according to, AlSRK14 construct was introduced in Col-0 and C24, 2006.

L. , Act:Venus construct was introduced in the selected AlSRK14 line #10. pACT11-RFP construct was introduced in the selected AlSCR14 line #4, C24

, LTI6b:GFP was introduced in Col-0. For all transformations, unique insertion lines homozygous for the

S. R. Cutler, D. W. Ehrhardt, J. S. Griffitts, and C. R. Somerville, Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency, Proceedings of the National Academy of Sciences, vol.97, pp.3718-3723, 2000.

E. Durand, R. Meheust, and M. Soucaze, Dominance hierarchy arising from the evolution of a complex small RNA regulatory network, Science, vol.346, pp.1200-1205, 2014.
URL : https://hal.archives-ouvertes.fr/hal-01110837

A. Era, M. Tominaga, K. Ebine, C. Awai, C. Saito et al.,

T. , Application of Lifeact Reveals F-Actin Dynamics in Arabidopsis thaliana and the Liverwort, Marchantia polymorpha, Plant and Cell Physiology, vol.50, pp.1041-1048, 2009.

I. Fobis-loisy, P. Chambrier, and T. Gaude, Genetic transformation of Arabidopsis lyrata: specific expression of the green fluorescent protein (GFP) in pistil tissues, Plant Cell Reports, vol.26, pp.745-753, 2007.
URL : https://hal.archives-ouvertes.fr/hal-00189103

R. M. Hackett, G. Cadwallader, and F. Franklin, Functional analysis of a Brassica oleracea SLR1 gene promoter, Plant physiology, vol.112, pp.1601-1607, 1996.

S. Huang, Y. An, J. M. Mcdowell, E. C. Mckinney, and R. B. Meagher, The Arabidopsis ACT11 actin gene is strongly expressed in tissues of the emerging inflorescence, pollen, and developing ovules, Plant molecular biology, vol.33, pp.125-139, 1997.

M. Karimi, D. Inzé, and A. Depicker, GATEWAY vectors for Agrobacterium-mediated plant transformation, Trends in Plant Science, vol.7, pp.193-195, 2002.

E. Logemann, R. P. Birkenbihl, B. Ülker, and I. E. Somssich, An improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation protocol, Plant Methods, vol.2, p.16, 2006.

N. Rotman, A. Durbarry, A. Wardle, W. C. Yang, A. Chaboud et al., A novel class of MYB factors controls sperm-cell formation in plants, Current Biology, vol.15, pp.244-248, 2005.
URL : https://hal.archives-ouvertes.fr/hal-00188852

N. Rotman, F. Rozier, L. Boavida, C. Dumas, F. Berger et al., Female control of male gamete delivery during fertilization in Arabidopsis thaliana, Current Biology, vol.13, pp.432-436, 2003.

D. Twell, R. Wing, J. Yamaguchi, and S. Mccormick, from stage 13-14E A. thaliana flower buds or twenty stigmas from A. lyrata buds just before anthesis were dissected and total RNA was extracted with the Arcturus® PicoPure® RNA isolation kit (Life Technologies). 265 ng of total RNA were reverse-transcribed with random hexanucleotides and RevertAid (200U/µL ; Thermo scientific) and subjected to quantitative real-time PCR with SRK14 or ACTIN8 specific primers. Because AlSRK14 expression level was compared between A. lyrata and A. thaliana, we designed primers that amplify a region of the ACTIN8 cDNA conserved between the two species, Molecular and General Genetics MGG, vol.217, pp.240-245, 1989.

, Amplification of SRK14 was performed with two primers located within the first exon (forward primer 5'-GCCGCCAGACACATCCGGGGC-3' and reverse primer 5

, Absence of contaminating genomic DNA was controlled with primers located within a non coding region close to the ACTIN8 gene (forward primer 5'-GGCTGTGACAATGCGAAGCCCC-3' and reverse primer 5'-CCCCATTTTGGTATCTAGGG-3'). Quantitative analysis of real-time PCR results was performed using the 2 -??Ct method, 2008.

T. D. Schmittgen and K. J. Livak, Analyzing real-time PCR data by the comparative C(T) method, Nature Protocols, vol.3, pp.1101-1108, 2008.

, Confocal Microscopy VENUS and GFP were excited at 488 nm and fluorescence detected between 450 and 550 nm, RFP and FM4-64 were excited at 561 nm and fluorescence detected between 550 and 600 nm, TURQUOISE was excited at 458 nm and fluorescence detected between 400 and 450 nm. To avoid emission spectrum overlay, image acquisition was performed with the sequential mode