1. « Pull down » pour recherche de l'interactant de la protéine recombinante ,
Mise en évidence d'interactions entre recProt 01230 et les fractions et du lysat cellulaire par « Far Western blot » ,
, Etude du rôle des glycosaminoglycanes dans l'adhésion d'E. ruminantium
Quantification du nombre de bactéries à chaque temps et des cDNA correspondants pour les ERGA_CDS_01230 et ERGA_CDS_05140 ,
, Evaluation du potentiel antigénique des protéines recProt 01230 et recProt 05140 d'E. ruminantium par
,
,
, Taille attendue et pureté élevée de la recProt 01230 et 05140
Présence des étiquettes GFP et Histidine sur recProt 01230 et 05140 ,
Présence de glycosylation sur recProt 01230 et 05140 ,
Prédiction des structures et localisation des protéines ERGA_CDS_ 01230 et 05140, p.45 ,
Mise en évidence de l'adhésion des protéines recombinantes avec les BAEC ,
Synchronisation de l'infection et croissance d'E. ruminantium in vitro ,
, IV. Réponse humorale associée aux protéines natives ERGA_CDS_01230 et 05140 lors d'essais vaccinaux avec le vaccin à bactéries atténuées ou inactivées
, Efficiency of inactivated vaccines against heartwater in Burkina Faso: impact of Ehrlichia ruminantium genetic diversity. Vaccine, vol.28, pp.4573-4580, 2010.
Trends in the control of heartwater, Onderstepoort J Vet Res, vol.76, pp.81-88, 2009. ,
Heartwater-Ehrlichia ruminantium infection, Rev Sci Tech, vol.34, pp.557-568, 2015. ,
Diverse functions of glycosaminoglycans in infectious diseases, Prog Mol Biol Transl Sci, vol.93, pp.373-394, 2010. ,
, Glycosaminoglycans and infection. Front Biosci, vol.21, pp.1260-1277, 2016.
, Glycobiology of syndecan-1 in bacterial infections, 2018.
Propagation of the tick Amblyomma variegatum in the Caribbean, Rev Sci Tech, vol.14, pp.841-855, 1995. ,
Proteoglycans in host-pathogen interactions: molecular mechanisms and therapeutic implications, Expert Rev Mol Med, vol.12, p.5, 2010. ,
Heparan Sulfate Proteoglycans in Infection, chapter II; Glycans in Diseases and Therapeutics, p.188, 2011. ,
Deciphering the protein interaction in adhesion of Francisella tularensis subsp. holarctica to the endothelial cells, Microb Pathog, vol.81, pp.6-15, 2015. ,
SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information, Nucleic Acids Res, vol.42, pp.252-258, 2014. ,
LEXSinduce 3 Expression Kit for inducible expression of recombinant proteins in Leishmania tarentolae, vol.28, 2011. ,
Bacterial entry into cells: a role for the endocytic machinery, FEBS Lett, vol.580, pp.2962-2967, 2006. ,
Listeria monocytogenes internalin and E-cadherin: from bench to bedside, Cold Spring Harb Perspect Biol, vol.1, p.3087, 2009. ,
Listeria monocytogenes internalin and E-cadherin: from structure to pathogenesis, Cell Microbiol, vol.11, pp.693-702, 2009. ,
2015: Parapatric distribution and sexual competition between two tick species, Amblyomma variegatum and A. hebraeum (Acari, Ixodidae), in Mozambique, Parasit Vectors, vol.8, p.504 ,
Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks, Parasit Vectors, vol.10, p.566, 2017. ,
Evolution and molecular mechanism of PitAs in iron transport of Streptococcus species, J Inorg Biochem, vol.182, pp.113-123, 2018. ,
Disruption of cadherin-related junctions triggers autocrine expression of vascular endothelial growth factor in bovine aortic endothelial cells : effects on cell proliferation and death resistance, Circ Res, vol.85, pp.1132-1138, 1999. ,
Rickettsial outermembrane protein B (rOmpB) mediates bacterial invasion through Ku70 in an actin, c-Cbl, clathrin and caveolin 2-dependent manner, Cell Microbiol, vol.11, pp.629-644, 2009. ,
O-linked glycosylation ensures the normal conformation of the autotransporter adhesin involved in diffuse adherence, J Bacteriol, vol.189, pp.8880-8889, 2007. ,
, The OmpA family of proteins: roles in bacterial pathogenesis and immunity, vol.163, pp.207-222, 2013.
MaxQuant enables high peptide identification rates, individualized p.p.b.range mass accuracies and proteome-wide protein quantification, Nat Biotechnol, vol.26, pp.1367-1372, 2008. ,
Adhesion of outer membrane proteins containing tandem repeats of Anaplasma and Ehrlichia species (Rickettsiales: Anaplasmataceae) to tick cells, Vet Microbiol, vol.98, pp.313-322, 2004. ,
Characterization of the functional domain of major surface protein 1a involved in adhesion of the rickettsia Anaplasma marginale to host cells, Vet Microbiol, vol.91, pp.265-283, 2003. ,
2012: alpha-Enolase, a multifunctional protein: its role on pathophysiological situations, J Biomed Biotechnol, p.156795, 2012. ,
Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila, Int J Syst Evol Microbiol, vol.51, pp.2145-2165, 2001. ,
Innovative approach for transcriptomic analysis of obligate intracellular pathogen: selective capture of transcribed sequences of Ehrlichia ruminantium, BMC Mol Biol, vol.10, p.111, 2009. ,
Conformation and orientation of a protein folding intermediate trapped by adsorption, Proc Natl Acad Sci U S A, vol.101, pp.11316-11321, 2004. ,
A glycosylated recombinant subunit candidate vaccine consisting of Ehrlichia ruminantium major antigenic protein1 induces specific humoral and Th1 type cell responses in sheep, PLoS One, vol.12, p.185495, 2017. ,
Glycosylation as a Main Regulator of Growth and Death Factor Receptors Signaling, Int J Mol Sci, vol.19, 2018. ,
Comparative genomics of three strains of Ehrlichia ruminantium: a review, Ann N Y Acad Sci, vol.1081, pp.417-433, 2006. ,
URL : https://hal.archives-ouvertes.fr/hal-00125031
Gateway-compatible vectors for high-throughput protein expression in proand eukaryotic cell-free systems, J Biotechnol, vol.195, pp.1-7, 2015. ,
, Current Protocols in Molecular-Biology-Ausubel,Fm. Nature, vol.333, pp.309-310, 1988.
Studying protein-protein interactions via blot overlay/far western blot, Methods Mol Biol, vol.1278, pp.371-379, 2015. ,
Anaplasma marginale Outer Membrane Protein A Is an Adhesin That Recognizes Sialylated and Fucosylated Glycans and Functionally Depends on an Essential Binding Domain, Infect Immun, p.85, 2017. ,
OmpA-mediated rickettsial adherence to and invasion of human endothelial cells is dependent upon interaction with alpha2beta1 integrin, Cell Microbiol, vol.15, pp.727-741, 2013. ,
A sequence property approach to searching protein databases, J Mol Biol, vol.251, pp.390-399, 1995. ,
Pour some sugar on it: the expanding world of bacterial protein O-linked glycosylation, Mol Microbiol, vol.89, pp.14-28, 2013. ,
Role of glycosaminoglycans in infectious disease, Methods Mol Biol, vol.1229, pp.567-585, 2015. ,
Synergy between the N-and C-terminal domains of InlB for efficient invasion of non-phagocytic cells by Listeria monocytogenes, Mol Microbiol, vol.42, pp.955-965, 2001. ,
Anaplasma phagocytophilum Asp14 is an invasin that interacts with mammalian host cells via its C terminus to facilitate infection, Infect Immun, vol.81, pp.65-79, 2013. ,
The Phyre2 web portal for protein modeling, prediction and analysis, Nat Protoc, vol.10, pp.845-858, 2015. ,
Production of glycosylated soluble amyloid precursor protein alpha (sAPPalpha) in Leishmania tarentolae, J Proteome Res, vol.12, pp.396-403, 2013. ,
Plasmodium falciparum BAEBL binds to heparan sulfate proteoglycans on the human erythrocyte surface, J Biol Chem, vol.285, pp.1716-1725, 2010. ,
Targeting the bacteria-host interface: strategies in anti-adhesion therapy, Virulence, vol.4, pp.284-294, 2013. ,
Outer membrane protein A and OprF: versatile roles in Gramnegative bacterial infections, FEBS J, vol.279, pp.919-931, 2012. ,
Obligatory intracellular parasitism by Ehrlichia chaffeensis and Anaplasma phagocytophilum involves caveolae and glycosylphosphatidylinositol-anchored proteins, Cell Microbiol, vol.5, pp.809-820, 2003. ,
, Borrelia burgdorferi glycosaminoglycan-binding proteins: a potential target for new therapeutics against Lyme disease. Microbiology, vol.163, pp.1759-1766, 2017.
Altered antibiotic transport in OmpC mutants isolated from a series of clinical strains of multi-drug resistant E. coli, PLoS One, vol.6, p.25825, 2011. ,
Sweet Talk: Protein Glycosylation in Bacterial Interaction With the Host, Trends Microbiol, vol.23, pp.630-641, 2015. ,
Cell surface adherence and endocytosis of protein transduction domains, Mol Ther, vol.8, pp.143-150, 2003. ,
A novel O-linked glycan modulates Campylobacter jejuni major outer membrane proteinmediated adhesion to human histo-blood group antigens and chicken colonization, Open Biol, vol.4, p.130202, 2014. ,
The historical introduction of cattle and #Amblyomma variegatum# in the French West Indies: bibliographic synthesis, 1998. ,
Tick-borne diseases in cattle: applications of proteomics to develop new generation vaccines, J Proteomics, vol.75, pp.4232-4250, 2012. ,
A user-friendly and scalable process to prepare a ready-touse inactivated vaccine: the example of heartwater in ruminants under tropical conditions, Vaccine, vol.33, pp.678-685, 2015. ,
Process development for the mass production of Ehrlichia ruminantium, Vaccine, vol.24, pp.1716-1725, 2006. ,
, Comparative Proteomic Profiling of Ehrlichia ruminantium Pathogenic Strain and Its High-Passaged Attenuated Strain Reveals Virulence and Attenuation-Associated Proteins, vol.10, 2015.
URL : https://hal.archives-ouvertes.fr/hal-01595370
Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures, Vet Microbiol, vol.110, pp.87-96, 2005. ,
Identification of OmpA, a Coxiella burnetii protein involved in host cell invasion, by multi-phenotypic high-content screening, PLoS Pathog, vol.10, p.1004013, 2014. ,
URL : https://hal.archives-ouvertes.fr/hal-01999429
2012: Invasion of the mammalian host : early events at the cellular and molecular levels : chapter IV, p.458 ,
Glycosylation of homologous immunodominant proteins of Ehrlichia chaffeensis and Ehrlichia canis, Infect Immun, vol.68, pp.13-18, 2000. ,
, Immunogenicity of Mycobacterium bovis BCG expressing Anaplasma marginale MSP1a antigen. Vaccine, vol.24, pp.6332-6339, 2006.
Escherichia coli electrotransformation, Methods Mol Biol, vol.47, pp.105-113, 1995. ,
Ehrlichia chaffeensis TRP120 effector targets and recruits host polycomb group proteins for degradation to promote intracellular infection, 2018. ,
EtpE Binding to DNase X Induces Ehrlichial Entry via CD147 and hnRNP-K Recruitment, Followed by Mobilization of N-WASP and Actin, MBio, vol.6, pp.1541-01515, 2015. ,
Ehrlichia chaffeensis uses its surface protein EtpE to bind GPI-anchored protein DNase X and trigger entry into mammalian cells, PLoS Pathog, vol.9, p.1003666, 2013. ,
Iron Starvation Conditions Upregulate Ehrlichia ruminantium Type IV Secretion System, tr1 Transcription Factor and map1 Genes Family through the Master Regulatory Protein ErxR, Front Cell Infect Microbiol, vol.7, p.535, 2017. ,
URL : https://hal.archives-ouvertes.fr/hal-01989584
Proteomic profiling of the outer membrane fraction of the obligate intracellular bacterial pathogen Ehrlichia ruminantium, PLoS One, vol.10, 2015. ,
URL : https://hal.archives-ouvertes.fr/hal-01203402
Ehrlichia's molecular tricks to manipulate their host cells, Microbes Infect, 2015. ,
2012: Recombinant protein production in the eukaryotic protozoan parasite Leishmania tarentolae: a review; 824, pp.307-315 ,
Leishmania tarentolae for the Production of Multi-subunit Complexes; 896, pp.155-165, 2016. ,
Anaplasma phagocytophilum outer membrane protein A interacts with sialylated glycoproteins to promote infection of mammalian host cells, Infect Immun, vol.80, pp.3748-3760, 2012. ,
, Quantification of Ehrlichia ruminantium by real time PCR, vol.107, pp.273-278, 2005.
DOI : 10.1016/j.vetmic.2005.02.001
Characterization of variable immunodominant antigens of Cowdria ruminantium by ELISA and immunoblots, Parasite Immunol, vol.20, pp.613-622, 1998. ,
Ehrlichia ruminantium infection (heartwater) in wild animals, Trends Parasitol, vol.18, pp.214-218, 2002. ,
DOI : 10.1016/s1471-4922(02)02251-1
Host cell-specific protein expression in vitro in Ehrlichia ruminantium, Vet Microbiol, vol.128, pp.136-147, 2008. ,
Protection against heartwater by DNA immunisation with four Ehrlichia ruminantium open reading frames, Vaccine, vol.25, pp.2316-2324, 2007. ,
Analyse du transcriptome d'Ehrlichia ruminantium agent causal de la cowdriose : mise en évidence des gènes impliqués dans la virulence et les mécanismes d'atténuation et application à l'élaboration d'un vaccin recombinant Guadeloupe, 2012. ,
Global gene expression profiling of Ehrlichia ruminantium at different stages of development, FEMS Immunol Med Microbiol, vol.64, pp.66-73, 2012. ,
Comparative Transcriptome Profiling of Virulent and Attenuated Ehrlichia ruminantium Strains Highlighted Strong Regulation of map1-and Metabolism Related Genes, Frontiers in Cellular and Infection Microbiology, vol.8, 2018. ,
Understanding Anaplasmataceae pathogenesis using, Omics" approaches. Front Cell Infect Microbiol, vol.4, p.86, 2014. ,
DOI : 10.3389/fcimb.2014.00086
URL : https://www.frontiersin.org/articles/10.3389/fcimb.2014.00086/pdf
Glycosaminoglycans are involved in bacterial adherence to lung cells, BMC Infect Dis, vol.17, p.319, 2017. ,
Mechanisms to create a safe haven by members of the family Anaplasmataceae, Ann N Y Acad Sci, vol.990, pp.548-555, 2003. ,
Molecular Pathogenesis of Ehrlichia chaffeensis Infection, Annu Rev Microbiol, vol.69, pp.283-304, 2015. ,
Vaccines and diagnostics for transboundary animal diseases, pp.17-19, 2012. ,
Fibroblast growth factor receptor-1 mediates internalization of pathogenic spotted fever rickettsiae into host endothelium, PLoS One, vol.12, p.183181, 2017. ,
Novel interactions of glycosaminoglycans and bacterial glycolipids mediate binding of enterococci to human cells, J Biol Chem, vol.284, pp.18194-18201, 2009. ,
Identification and Characterization of a Candidate Wolbachia pipientis Type IV Effector That Interacts with the Actin Cytoskeleton, p.7, 2016. ,
High resolution structure of an alternate form of the ferric ion binding protein from Haemophilus influenzae, J Biol Chem, vol.278, pp.11513-11519, 2003. ,
Protein folding and misfolding on surfaces, Int J Mol Sci, vol.9, pp.2515-2542, 2008. ,
Economic Game Theory to Model the Attenuation of Virulence of an Obligate Intracellular Bacterium, Front Cell Infect Microbiol, vol.6, p.86, 2016. ,
URL : https://hal.archives-ouvertes.fr/hal-01601401
UniProt: the universal protein knowledgebase, Nucleic Acids Res, vol.45, pp.158-169, 2017. ,
Anion-independent iron coordination by the Campylobacter jejuni ferric binding protein, J Biol Chem, vol.280, pp.9283-9290, 2005. ,
Cowdriose, chapitre 2.1.9; Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, p.7, 2017. ,
Opportunities in Diagnostic and vaccine approaches to mitigate potential heartwater spreading and impact on the american mainland. Vaccines and diagnostics for transboundary animal diseases, Dev Biol, vol.135, pp.191-200, 2013. ,
Sugar and Spice Make Bacteria Not Nice: Protein Glycosylation and Its Influence in Pathogenesis, J Mol Biol, vol.428, pp.3206-3220, 2016. ,
, <Ticks of Domestic Animals in Africa a guide_Walker_2014.pdf>, 2003.
2015: I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Res, vol.43, pp.174-181 ,
Prediction of protein subcellular localization, Proteins, vol.64, pp.643-651, 2006. ,