M. Magistrini, Advances in cooled semen technology, Anim. Reprod. Sci, vol.68, pp.181-190, 2001.

D. Beebe, M. Wheeler, H. Zerigue, E. R. Walters, and S. , Microfluidic technology for assisted reproduction, Theriogenology, vol.57, pp.125-135, 2002.

M. Benchaib, V. Braun, J. Lornage, S. Hadj, B. Salle et al., DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique, 2003.

E. Bianchi, W. , and G. J. , Izumo meets Juno: preventing polyspermy in fertilization, Cell Cycle, vol.13, pp.2019-2020, 2014.

E. Bianchi, B. Doe, D. Goulding, W. , and G. J. , Juno is the egg Izumo receptor and is essential for mammalian fertilization, Nature, vol.508, pp.483-487, 2014.

J. F. Bilodeau, S. Blanchette, C. Gagnon, and M. A. Sirard, Thiols preventsH2O2mediated loss of sperm motility in cryopreserved bull semen, Theriogenology, 2001.

J. F. Bilodeau, S. Blanchette, N. Cormier, and M. A. Sirard, Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase

S. Bilodeau-goeseels, Bovine oocyte meiotic inhibition before in vitro maturation and its value to in vitro embryo production: does it improve developmental competence?, Reprod. Domest. Anim, vol.47, pp.687-693, 2012.

N. Bissonnette, J. P. Lévesque-sergerie, C. B. Thibault, and G. , Spermatozoal transcriptome profiling for bull sperm motility: A potential tool to evaluate semen quality, Reproduction, vol.138, pp.65-80, 2009.

P. F. Blackmore, J. Neulen, F. Lattanzio, and S. J. Beebe, Cell surface-binding sites for progesterone mediate calcium uptake in human sperm, J. Biol. Chem, vol.266, pp.18655-18659, 1991.

J. D. Bleil and P. M. Wassarman, Sperm-egg interactions in the mouse: sequence of events and induction of the acrosome reaction by a zona pellucida glycoprotein, Dev. Biol, vol.95, pp.317-324, 1983.

P. Blondin, M. Beaulieu, V. Fournier, N. Morin, L. Crawford et al., Analysis of bovine sexed sperm for IVF from sorting to the embryo, Theriogenology, vol.71, pp.30-38, 2009.

G. Bo and R. Mapletoft, Evaluation and classification of bovine embryos, 2013.

T. Borggrefe, L. , and R. , Fine-tuning of the intracellular canonical Notch signaling pathway, Cell Cycle, vol.11, pp.264-276, 2012.

S. Boryshpolets, S. Pérez-cerezales, and M. Eisenbach, Behavioral mechanism of human sperm in thermotaxis: a role for hyperactivation, Hum. Reprod, vol.30, pp.884-892, 2015.

K. S. Bose and R. H. Sarma, Relationships between the dynamics of iatrogenic DNA damage and genomic design in mammalian spermatozoa from eleven species, Biochem. Biophys. Res. Commun, vol.66, pp.951-961, 1975.

A. Graf, S. Krebs, V. Zakhartchenko, B. Schwalb, H. W. Blum et al., Fine mapping of genome activation in bovine embryos by RNA sequencing, Proc. Natl. Acad. Sci. USA, vol.111, pp.4139-4144, 2014.

A. Graf, S. Krebs, M. Heininen-brown, V. Zakhartchenko, H. W. Blum et al., , 2014.

, Genome activation in bovine embryos: Review of the literature and new insights from RNA sequencing experiments, Anim. Reprod. Sci, vol.149

H. D. Guthrie, W. , and G. R. , Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in percoll-treated viable boar sperm using fluorescenceactivated flow cytometry, J Anim Sci, vol.84, pp.2089-100

S. Hamamah and J. L. Gatti, Role of the ionic environment and internal pH on sperm activity, Hum. Reprod, vol.13, pp.20-30, 1998.

R. H. Hammerstedt, J. K. Graham, N. , and J. P. , Cryopreservation of mammalian sperm: what we ask them to survive, J. Androl, vol.11, pp.73-88, 1990.

R. H. Hammerstedt, J. K. Graham, N. , and J. P. , Cryopreservation ofmammalian sperm: what we ask them to survive, J. Androl, pp.11-73, 1990.

H. M. Harshan, S. Sankar, L. P. Singh, M. K. Singh, S. Sudharani et al., Identification of PDC-109-like protein(s) in buffalo seminal plasma, Anim. Reprod, vol.115, pp.306-311, 2009.

A. Herrler, S. Eisner, V. Bach, U. Weissenborn, and H. M. Beier, Cryopreservation of spermatozoa in alginic acid capsules, Fertil Steril, 2006.

H. C. Ho, K. A. Granish, and S. S. Suarez, Hyperactivated motility of bull sperm is triggered at the axoneme by ca2þ and not camp, Dev Biol), 2002.

E. K. Hodgson and I. Fridovich, The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: inactivation of the enzyme, Biochemistry, vol.14, pp.5294-5299, 1975.

D. A. Hoganson and D. P. Stahly, Regulation of dihydrodipicolinate synthase during growth and sporulation of Bacillus cereus, J. Bacteriol, vol.124, pp.1344-1350, 1975.

J. H. Hu, L. S. Zan, X. L. Zhao, Q. W. Li, Z. L. Jiang et al., of trehalose supplementation on semen quality and oxida-tive stress variables in frozen-thawed bovine semen, J. Anim. Sci, pp.88-1657, 2010.

P. Hyttel, H. Callesen, G. , and T. , A comparative ultrastructural study of in vivo versus in vitro fertilization of bovine oocytes, Anat. Embryol, vol.179, pp.435-442, 1989.

D. Ickowicz, M. Finkelstein, and H. Breitbart, Mechanism of sperm capacitation and the acrosome reaction: role of protein kinases, Asian J. Androl, vol.14, pp.816-821, 2012.

K. Inaba, Molecular basis of sperm flagellar axonemes: structural and evolutionary aspects, Ann. N. Y. Acad. Sci, vol.1101, pp.506-526, 2007.

K. Inaba and K. Mizuno, Sperm dysfunction and ciliopathy, Reprod. Med. Biol, vol.15, pp.77-94, 2016.

N. Inoue, M. Ikawa, A. Isotani, and M. Okabe, The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs, Nature, vol.434, pp.234-238, 2005.

N. Inoue, Y. Satouh, M. Ikawa, M. Okabe, Y. et al., Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs, Proc. Natl. Acad. Sci. U.S.A, vol.108, 2011.

N. Inoue, D. Hamada, H. Kamikubo, K. Hirata, M. Kataoka et al., Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion, Development, vol.140, pp.3221-3229, 2013.

S. Ishijima, Ca2+ and cAMP regulations of microtubule sliding in hyperactivated motility of bull spermatozoa, Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci, vol.91, pp.99-108, 2015.

M. Jin, E. Fujiwara, Y. Kakiuchi, M. Okabe, Y. Satouh et al., Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization, Proc. Natl. Acad. Sci. U.S.A, vol.108, pp.4892-4896, 2011.

J. , D. Cr, M. Ma, L. Jf, M. Rl et al., Effects of 2.1 and 3.5x10(6) sex-sorted sperm dosages on conception rates of Holstein cows and heifers, J Dairy Sci

L. A. Johnson, Sexing mammalian sperm for production of offspring: The state-of-theart, Anim. Reprod. Sci, vol.60, pp.93-107, 2000.

J. Kalous, A. Tetkova, M. Kubelka, and A. Susor, Importance of ERK1/2 in Regulation of Protein Translation during Oocyte Meiosis, Int J Mol Sci, 2018.

V. Kantsler, J. Dunkel, M. Blayney, and R. E. Goldstein, Correction: Rheotaxis facilitates upstream navigation of mammalian sperm cells, vol.3, p.3521, 2014.

R. Kasimanickam, V. Kasimanickam, A. Tibary, and K. Pelzer, , 2011.

U. B. Kaupp, 100 years of sperm chemotaxis, J. Gen. Physiol, vol.140, pp.583-586, 2012.

U. B. Kaupp, J. Solzin, E. Hildebrand, J. E. Brown, A. Helbig et al., The signal flow and motor response controling chemotaxis of sea urchin sperm, Nat. Cell Biol, vol.5, pp.109-117, 2003.

M. Knowlton, M. A. Sadasivam, and S. , Microfluidics for sperm research, Trends Biotech, vol.4, 2015.

H. Kodama, Y. Kuribayashi, and C. Gagnon, Effect of sperm lipidperoxidation on fertilization, J. Androl, vol.17, pp.151-157, 1996.

E. De-lamirande, A. Harakat, and C. Gagnon, Human sperm capacitation induced by biological fluids and progesterone, but not by NADH or NADPH, 1998.

P. Mazur, N. Katkova, and J. K. Critser, The enhancement of the ability of mouse sperm to survive freezing and thawing by the use of high concentrations of glycerol and the presence of an Escherichia coli membrane preparation

E. S. Mcneil, Nanotechnology for the biologist, J. Leukoc. Biol, vol.78, pp.1-10, 2005.

C. M. Medeiros, F. Forell, A. T. Oliveria, and J. L. Rodrigues, Currentstatus of sperm cryopreservation: why isn, 2002.

E. Memili, T. Dominko, and N. L. First, Onset of transcription in bovine oocytes and preimplantation embryos, Mol. Reprod. Dev, vol.51, pp.36-41, 1998.

K. Miki, W. D. Willis, P. R. Brown, E. H. Goulding, K. D. Fulcher et al., Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility, Dev. Biol, vol.248, pp.331-342, 2002.

D. Miller, Sperm RNA as a mediator of genomic plasticity. Article ID 179701, p.13, 2014.

M. Miyashita, J. Otsuki, Y. Hanai, Y. Nakagawa, and H. Miyagawa, Characterization of peptide components in the venom of the scorpion Liocheles australasiae (Hemiscorpiidae), 2007.

, Toxicon, vol.50, pp.428-437

J. M. Mora, M. A. Fenwick, L. Castle, M. Baithun, T. A. Ryder et al., Characterization and significance of adhesion and junction-related proteins in mouse ovarian follicles, Biol. Reprod, vol.86, pp.1-14, 2012.

R. Muino, M. Fernandez, and A. I. Pena, Post-thaw survival andlongevity of bull spermatozoa frozen with an egg yolk-based or twoegg yolk-free extenders after an equilibration period of 18 h, Reprod.Domest. Anim, vol.42, pp.305-311, 2007.

K. Muller, T. Pomorski, P. Muller, H. , and A. , Stability oftransbilayer phospholipid asymmetry in viable ram sperm cells aftercryotreatment, J. Cell Sci, vol.112, pp.11-20, 1999.

M. Myers, K. L. Britt, N. G. Wreford, F. J. Ebling, and J. B. Kerr, Methods for quantifying follicular numbers within the mouse ovary, Reproduction, vol.127, pp.569-580, 2004.

S. Narisawa, N. B. Hecht, E. Goldberg, K. M. Boatright, J. C. Reed et al., , 2002.

, Testis-specific cytochrome c-null mice produce functional sperm but undergo early testicular atrophy, Mol. Cell. Biol, vol.22, pp.5554-5562

A. Nath, V. Sharma, P. K. Dubey, M. D. Pratheesh, N. E. Gade et al., Impact of gonadotropin supplementation on the expression of germ cell marker genes, Biol. Anim, vol.49, pp.34-41, 2013.

B. Navarro, Y. Kirichok, J. Chung, and D. E. Clapham, Ion channels that control fertility in mammalian spermatozoa, Int. J. Dev. Biol, vol.52, pp.607-613, 2008.

A. E. Newell, S. E. Fiedler, J. M. Ruan, J. Pan, P. J. Wang et al., Protein kinase A RII-like (R2D2) proteins exhibit differential localization and AKAP interaction, Cell Motil. Cytoskeleton, vol.65, pp.539-552, 2008.

H. K. Niemann and W. A. , Application of transgenesis in livestock for agriculture and biomedicine, Anim.Reprod. Sci, vol.79, pp.291-317, 2003.

H. W. Niemann and C. , Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: Implications for subsequent development, Theriogenology, vol.53, pp.21-34, 2000.

M. Nieto, E. Muñoz, J. Carreira, and J. M. Andreu, Conformational and molecular responses to pH variation of the purified membrane adenosine triphosphatase of Micrococcus lysodeikticus, Biochim. Biophys. Acta, vol.413, pp.394-414, 1975.

H. D. Norman, J. L. Hutchison, and R. H. Miller, Use of sexed semen and its effect on conception rate, calf sex, dystocia, and stillbirth of Holsteins in the United States, J. Dairy Sci, vol.93, pp.3880-3890, 2010.

J. Y. Nothias, S. Majumder, K. J. Kaneko, and M. L. Depamphilis, Regulation of gene expression at the beginning of mammalian development, J. Biol. Chem, vol.270, pp.22077-22080, 1995.

R. Oko, Comparative analysis of proteins from the fibrous sheath and outer dense fibers of rat spermatozoa, Biol. Reprod, vol.39, pp.169-182, 1988.

F. Otsuka, Z. Yao, T. Lee, S. Yamamoto, G. F. Erickson et al., Bone morphogenetic protein-15. Identification of target cells and biological functions, J. Biol. Chem, vol.275, pp.39523-39528, 2000.

F. Otsuka, K. J. Mctavish, and S. Shimasaki, Integral role of GDF-9 and BMP-15 in ovarian function, Mol. Reprod. Dev, vol.78, pp.9-21, 2011.

P. K. Pankaj, Study of Critical Control Points Associated withCryopreservation of Bovine Semen, 2006.

J. E. Parks, G. , and J. K. , Effects of cryopreservation procedures onsperm membranes, Theriogen, vol.38, pp.209-222, 1992.

J. E. Parks and D. V. Lynch, Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes (Cryobiology), 1992.

J. J. Parrish, J. Susko-parrish, M. A. Winer, and N. L. First, Capacitation of bovine sperm by heparin, Biol. Reprod, vol.38, pp.1171-1180, 1988.

J. J. Parrish, J. L. Susko-parrish, G. , and J. K. , In vitro capacitation ofbovine spermatozoa: role of intracellular calcium, pp.51-461, 1999.

W. H. Parry, F. Martorano, and E. K. Cotton, Management of life-threatening asthma with intravenous isoproterenol infusions, Am. J. Dis. Child, vol.130, pp.39-42, 1976.

D. Peddinti, B. Nanduri, A. Kaya, J. M. Feugang, C. B. Shane et al., , 2008.

, Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility, BMC Syst. Biol, vol.2, p.19

R. Pereira, R. Sá, A. Barros, and M. Sousa, Major regulatory mechanisms involved in sperm motility, Asian J Androl, vol.19, pp.5-14, 2017.

S. D. Perreault, R. , and B. J. , Capacitation pattern of human spermatozoa, Fertil. Steril, vol.38, pp.258-260, 1982.

S. Perteghella, A. Gaviraghi, S. Cenadelli, V. Bornaghi, A. Galli et al., Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation, J Vet Sci, vol.18, issue.1, pp.81-88, 2017.

S. Perteghella, B. Vigani, B. Crivelli, M. Spinaci, G. Galeati et al., Sperm Encapsulation from 1985 to Date, Technology Evolution and New Challenges in Swine Reproduction, pp.98-102, 2015.

D. L. Peterson, J. M. Gleisner, and R. L. Blakley, Bovine liver dihydrofolate reductase: purification and properties of the enzyme, Biochemistry, vol.14, pp.5261-5267, 1975.

D. M. Phillips, Mitochondrial disposition in mammalian spermatozoa, J. Ultrastruct. Res, pp.144-154, 1977.

E. O. Pietrobon, M. Monclus, A. De-los, A. J. Alberdi, and M. W. Fornés, Progesterone receptor availability in mouse spermatozoa during epididymal transit and capacitation: ligand blot detection of progesterone-binding protein, J. Androl, vol.24, pp.612-620, 2003.

R. H. Ponce, U. A. Urch, Y. , and R. , Inhibition of sperm-egg fusion in the hamster and mouse by carbohydrates, Zygote, vol.2, pp.253-262, 1994.

S. J. Publicover and C. L. Barratt, Sperm motility: things are moving in the lab!, Mol. Hum. Reprod, vol.17, pp.453-456, 2011.

S. H. Purcell, M. M. Chi, S. Lanzendorf, and K. H. Moley, Insulin-stimulated glucose uptake occurs in specialized cells within the cumulus oocyte complex, Endocrinology, vol.153, pp.2444-2454, 2012.

V. Quintero-hernández, S. Ramírez-carreto, M. T. Romero-gutiérrez, L. L. Valdez-velázquez, B. Becerril et al., Transcriptome analysis of scorpion species belonging to the Vaejovis genus, PLoS ONE, vol.10, 2015.

R. Rajhans, G. S. Kumar, P. K. Dubey, and G. T. , Effect of timing of development on total cell number and expression profile of HSP-70.1 and GLUT-1 in buffalo (Bubalus bubalis) oocytes and preimplantation embryos produced in vitro, Cell Biol. Int, vol.34, pp.463-468, 2010.

K. B. Rao, C. H. Pawshe, and S. M. , Sex determination of in vitro develoeped buffalo (Bubalus bubalis) embryoes by DNA amplification, Mol. Reprod. Dev, vol.36, pp.291-296, 1993.

B. Ravaux, N. Garroum, E. Perez, H. Willaime, and C. Gourier, A specific flagellum beating mode for inducing fusion in mammalian fertilization and kinetics of sperm internalization, Sci Rep, vol.6, p.31886, 2016.
URL : https://hal.archives-ouvertes.fr/hal-02128572

S. Reith and S. Hoy, Review: Behavioral signs of estrus and the potential of fully automated systems for detection of estrus in dairy cattle, Animal, vol.12, pp.398-407, 2018.

B. Renaud, M. Buda, B. D. Lewis, and J. F. Pujol, Effects of 5,6-dihydroxytryptamine on tyrosine-hydroxylase activity in central catecholaminergic neurons of the rat, Biochem. Pharmacol, vol.24, pp.1739-1742, 1975.

K. Reynaud, Le dialogue ovocyte-granulosa au cours de la folliculogenèse, Médecine Thérapeutique / Endocrinologie, vol.5, pp.85-93, 2003.

F. J. Richard, Regulation of meiotic maturation, J. Anim. Sci, vol.85, pp.4-6, 2007.

J. I. Rishpon and D. , An amperometric enzyme channeling immunosensor, Biosens, vol.12, pp.195-204, 1997.

R. , N. , R. G. , and S. , Technology and applications for encapsulated spermatozoa, Biotechnol Adv, vol.1

L. Robertson, J. L. Bailey, and M. M. Buhr, Effects of cold shock andphospholipase A2on intact boar spermatozoa and sperm headplasma membranes, Mol. Reprod. Dev, vol.26, pp.143-149, 1990.

J. M. Robl, Z. Wang, P. K. Kasinthan, and Y. , Transgenic animal production and animal biotechnology, Theriogeneology, vol.67, pp.127-133, 2007.

E. R. Roldan, T. Murase, and Q. X. Shi, Exocytosis in spermatozoa in response to progesterone and zona pellucida, Science, vol.266, pp.1578-1581, 1994.

M. Rossato, L. Sala, G. B. Balasini, M. Taricco, F. Galeazzi et al., Sperm treatment with extracellular ATP increases fertilization rates in in-vitro fertilization for male factor infertility, Hum. Reprod, vol.14, pp.694-697, 1999.

A. M. Salicioni, M. D. Platt, E. V. Wertheimer, E. Arcelay, A. Allaire et al., Signalling pathways involved in sperm capacitation, Soc Reprod Fertil Suppl, vol.65, pp.245-259, 2007.

J. C. Samper, J. C. Hellander, and B. G. Crabo, Relationship between the fertility of fresh and frozen stallion semen and semen quality, J Reprod Fertil, 1991.

S. Sar?özkan, M. N. Bucak, P. B. Tuncer, P. A. Ulutas¸, and A. Bilgen, influence of cysteine and taurine on microscopic-oxidative stressparameters and fertilizing ability of bull semen following cryopreser-vation, Cryobiology, vol.58, pp.134-138, 2009.

C. M. Saunders, M. G. Larman, J. Parrington, L. J. Cox, J. Royse et al., PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development, Development, vol.129, pp.3533-3544, 2002.

G. E. Seidel and . Jr, Update on sexed semen technology in cattle, Animal, vol.8, pp.160-164, 2014.

N. L. Selokar, M. Saini, P. Palta, M. S. Chauhan, R. S. Manik et al., Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen, PLoS One, vol.9, 2014.

G. Seydoux and R. E. Braun, Pathway to totipotency: lessons from germ cells, Cell, vol.127, pp.891-904, 2006.

R. A. Shah, A. George, M. K. Singh, D. Kumar, M. S. Chauhan et al., Hand-made cloned buffalo (Bubalus bubalis) embryos: Comparison of different media and culture systems, Cloning Stem Cells, vol.10, pp.435-442, 2008.

R. A. Shah, A. George, M. K. Singh, D. Kumar, T. Anand et al., Pregnancies established from handmade cloned blastocysts reconstructed using skin fibroblasts in buffalo (Bubalus bubalis), Theriogenology, vol.71, pp.1215-1219, 2009.

A. K. Shahiduzzaman and C. Linde-forsberg, Induced immotility during long-term storage at +5 degrees C does not prolong survival of dog spermatozoa, Theriogenology, vol.68, pp.920-933, 2007.

X. Shao, H. A. Tarnasky, U. Schalles, R. Oko, and F. A. Van-der-hoorn, Interactional cloning of the 84-kDa major outer dense fiber protein Odf84. Leucine zippers mediate associations of Odf84 and Odf27, J. Biol. Chem, vol.272, pp.6105-6113, 1997.

G. T. Sharma, A. Nath, S. Prasad, S. Singhal, N. Singh et al., Expression and characterization of constitutive heat shock protein 70.1 (HSPA-1A) gene in in vitro produced and in vivo-derived buffalo (Bubalus bubalis) embryos, Reprod. Dom. Anim, vol.47, pp.975-983, 2012.

T. J. Sheldon, I. Miguel-aliaga, A. P. Gould, W. R. Taylor, and D. Conklin, A novel family of single VWC-domain proteins in invertebrates, FEBS Lett, vol.581, pp.5268-5274, 2007.

A. K. Singh, V. K. Singh, B. M. Narwade, T. K. Mohanty, and S. K. Atreja, quality assessment of buffalo (Bubalus bubalis) semenchilled (5?C) in egg yolk-and soya milk-based extenders, Reprod.Domest. Anim, vol.47, pp.596-600, 2012.

S. K. Singla, N. L. Selokar, M. Saini, P. Palta, M. S. Chauhan et al., Buffalo cloning: What we have achieved so far, Curr. Sci, vol.109, pp.670-671, 2015.

J. E. Smitz and R. G. Cortvrindt, The earliest stages of folliculogenesis in vitro, Reproduction, vol.123, pp.185-202, 2002.

S. K. Sood, M. S. Chauhan, and O. S. , A direct duplex PCR assay for sex determination of Murrah buffalo embryos, Buffalo J, vol.17, pp.113-124, 2001.

J. Srivastava, S. Premi, S. A. Kumar, and S. , Organization and differential expression of the GACA/GATA tagged somatic and spermatozoal transcriptomes in buffalo Bubalus bubalis, BMC Genomics, vol.9, p.132, 2008.

N. Srivastava, A. Jerome, S. K. Srivastava, S. K. Ghosh, and A. , Bovine seminal PDC-109 protein: An overview of biochemical and functional properties, Anim. Reprod. Sci, vol.138, pp.1-13, 2013.

D. St-johnston, The intracellular localization of messenger RNAs, Cell, vol.81, pp.161-170, 1995.

F. B. Standerholen, K. E. Waterhouse, A. G. Larsgard, R. T. Garmo, F. D. Myromslien et al., Use of immobilized cryopreserved bovine semen in a blind artificial insemination trial, Theriogenology, vol.84, pp.413-420, 2015.

G. G. Vajta and M. , Science and technology of farm animal cloning: State of the art, Anim. Reprod. Sci, vol.92, pp.211-230, 2006.

O. P. Verma, R. Kumar, A. Kumar, and S. , Assisted reproductive techniques in farm animal-From artificial insemination to nanobiotechnology, Vet. World, vol.5, pp.301-310, 2012.

V. Verma, S. K. Gautam, P. Palta, R. S. Manik, S. K. Singla et al., , 2008.

, Development of a pronuclear DNA microinjection technique for production of green fluorescent protein-expressing bubaline (Bubalus bubalis) embryos, Theriogenology, vol.69, pp.655-665

P. E. Visconti, X. Ning, M. W. Fornés, J. G. Alvarez, P. Stein et al., Cholesterol efflux-mediated signal transduction in mammalian sperm: cholesterol release signals an increase in protein tyrosine phosphorylation during mouse sperm capacitation, Dev. Biol, vol.214, pp.429-443, 1999.

P. E. Visconti, D. Krapf, J. L. De-la-vega-beltrán, J. J. Acevedo, D. et al., Ion channels, phosphorylation and mammalian sperm capacitation, Asian J. Androl, vol.13, pp.395-405, 2011.

R. Vishwanath, Artificial insemination: the state of the art, Theriogenology, vol.59, pp.571-584, 2003.

R. J. Wall, Transgenic livestock: Progress and prospects for the future, Theriogenology, vol.45, pp.57-68, 1996.

C. Wambergue, R. Zouari, S. Fourati-ben-mustapha, G. Martinez, F. Devillard et al., Patients with multiple morphological abnormalities of the sperm flagella due to DNAH1 mutations have a good prognosis following intracytoplasmic sperm injection, Hum. Reprod, vol.31, pp.1164-1172, 2016.

H. M. Warriach, D. M. Mcgill, R. D. Bush, P. C. Wynn, and K. R. , A review of recent developments in buffalo reproduction-A review, Asian Aust. J. Anim. Sci, vol.28, pp.451-455, 2015.

P. F. Watson, Recent developments and concepts in thecryopreservation of spermatozoa and the assessment of theirpost-thawing function, Reprod. Fertil. Dev, vol.7, pp.871-891, 1995.

P. F. Watson, The causes of reduce fertility with cryopreservedsemen, Anim. Reprod. Sci, vol.60, pp.481-492, 2000.

D. J. Wells, Genetically modified animals and pharmacological research, Handb. Exp, vol.199, pp.213-216, 2010.

T. S. Witte and S. Schäfer-somi, Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa, Anim. Reprod. Sci, vol.102, pp.181-193, 2007.

X. Yue, C. Dechow, and W. Liu, A limited number of Y chromosome lineages is present in North American Holsteins, J. Dairy Sci, vol.98, pp.2738-2745, 2015.

N. Zanetti and L. S. Mayorga, Acrosomal swelling and membrane docking are required for hybrid vesicle formation during the human sperm acrosome reaction, Biol. Reprod, vol.81, p.396, 2009.

, Molecular Human Reproduction, vol.23, issue.2, pp.116-131, 2017.

, /molehr/gaw075 ORIGINAL ARTICLE Spermaurin, an La1-like peptide from the venom of the scorpion Scorpio maurus palmatus, improves sperm motility and fertilization in different mammalian species, Advanced Access publication on December, vol.28, 2016.

, Eric Schmitt 1 , Philippe Bulet 2,3,4 , and Christophe Arnoult 2,3,* 1 IMV Technologies, ZI N° 1 Est, F-61300 L'Aigle, France 2 Université Grenoble Alpes, Grenoble F-38000, France 3 Institute for Advanced Biosciences INSERM U1209, CNRS UMR5309, Grenoble F-38000, France 4 Plateforme BioPark d'Archamps, 5 L'institut du thorax, vol.2, 2016.

;. Montroeul-au-bois, M. A. Milford, and U. ). , Separation was performed on an Accucore C 18 column (2.1 mm × 150 mm from Thermo Scientific, Bremen, Germany), maintained at 35°C and online coupled to a Thermo Q-Exactive Orbitrap. Solvent A was 0.1% formic acid and solvent B was ACN in 0.1% formic acid. A linear gradient, starting from 4% B to 60% B for 120 min at a flow rate of 350 µl/min was applied to fractionate the venom components. The high-resolution mass spectrometer was operating in positive polarity and data-dependent mode. The Q-Exactive Orbitrap acquired a full-range scan from 380 to 2000 Th (70 000 resolution, AGC target 3.10 6 , maximum IT 200 ms) and then fragmented the top 10 peptide ions in each cycle (17 500 resolution, AGC target 10 5 , maximum IT 100 ms, intensity threshold 2 × 10 4 , excluding charge-unassigned ions, Normalized Collision Energy selected at 27), Ten milligram of lyophilized crude venom was dissolved in 500 µl of 100 mM ammonium acetate pH 6.8 and centrifuged at 10 000g for 10 min. Two hundred and fifty microliter of supernatant were used for further purifications. Purification by size exclusion chromatography Separation was performed on a Superdex peptide 10/300 GL gel filtration column (GE Healthcare

M. A. Abdel-rahman, V. Quintero-hernandez, and L. D. Possani, Venom proteomic and venomous glands transcriptomic analysis of the Egyptian scorpion Scorpio maurus palmatus (Arachnida: Scorpionidae), Toxicon, vol.74, pp.193-207, 2013.

R. Abi-nahed, J. Escoffier, C. Revel, L. Jeammet, C. Payre et al., The effect of group X secreted phospholipase A2 on fertilization outcome is specific and not mimicked by other secreted phospholipases A2 or progesterone, Biochimie, vol.99, pp.88-95, 2014.

R. J. Aitken, A. Mattei, and S. Irvine, Paradoxical stimulation of human sperm motility by 2-deoxyadenosine, J Reprod Fertil, vol.78, pp.515-527, 1986.

S. M. Curi, J. I. Ariagno, P. H. Chenlo, G. R. Mendeluk, M. N. Pugliese et al., Asthenozoospermia: analysis of a large population, Arch Androl, vol.49, pp.343-349, 2003.

D. Silva, P. Strzepa, A. Jouvensal, L. Rahioui, I. Gressent et al., A folded and functional synthetic PA1b: an interlocked entomotoxic miniprotein, Biopolymers, vol.92, pp.436-444, 2009.
URL : https://hal.archives-ouvertes.fr/hal-00521878

M. J. Davies, V. M. Moore, K. J. Willson, E. P. Van, K. Priest et al., Reproductive technologies and the risk of birth defects, N Engl J Med, vol.366, pp.1803-1813, 2012.

T. E. De, N. J. Aparicio, D. Turner, and L. Schwarzstein, Effect of two phosphodiesterase inhibitors, cyclic adenosine 3':5'-monophosphate, and a betablocking agent on human sperm motility, Fertil Steril, vol.29, pp.328-331, 1978.

C. Derache, H. Meudal, V. Aucagne, K. J. Mark, M. Cadene et al., Initial insights into structure-activity relationships of avian defensins, J Biol Chem, vol.287, pp.7746-7755, 2012.
URL : https://hal.archives-ouvertes.fr/hal-00721842

D. Peigneur, S. Clynen, E. Marien, T. Czech, L. Schoofs et al., Molecular diversity of the telson and venom components from Pandinus cavimanus (Scorpionidae Latreille 1802): transcriptome, venomics and function, Proteomics, vol.12, pp.313-328, 2012.

J. Escoffier, M. Couvet, H. De-pomyers, P. F. Ray, M. Sève et al., Snake venoms as a source of compounds modulating sperm physiology: secreted phospholipases A2 from Oxyuranus scutellatus scutellatus impact sperm motility, acrosome reaction and in vitro fertilization in mice, Biochimie, vol.92, pp.826-836, 2010.
URL : https://hal.archives-ouvertes.fr/hal-00497569

J. Escoffier, V. Pierre, I. Jemel, L. Munch, Z. Boudhraa et al., Group X secreted phospholipase A2 specifically decreases sperm motility in mice, J Cell Physiol, vol.226, pp.2601-2609, 2011.
URL : https://hal.archives-ouvertes.fr/hal-00731431

M. Essig, C. Schoenfeld, R. D. Amelar, L. Dubin, and G. Weiss, Stimulation of human sperm motility by relaxin, Fertil Steril, vol.38, pp.339-343, 1982.

K. Fosgerau and T. Hoffmann, Peptide therapeutics: current status and future directions, Drug Discov Today, vol.20, pp.122-128, 2015.

M. Gongora-benitez, J. Tulla-puche, and F. Albericio, Multifaceted roles of disulfide bonds. Peptides as therapeutics, Chem Rev, vol.114, pp.901-926, 2014.

D. G. Hammitt, E. Bedia, P. R. Rogers, C. H. Syrop, J. F. Donovan et al., Comparison of motility stimulants for cryopreserved human semen, Fertil Steril, vol.52, pp.495-502, 1989.

A. L. Harvey, Toxins and drug discovery, Toxicon, vol.92, pp.193-200, 2014.

B. Kovacic, V. Vlaisavljevic, and M. Reljic, Clinical use of pentoxifylline for activation of immotile testicular sperm before ICSI in patients with azoospermia, J Androl, vol.27, pp.45-52, 2006.

F. Lanzafame, M. G. Chapman, A. Guglielmino, C. M. Gearon, and R. G. Forman, Pharmacological stimulation of sperm motility, Hum Reprod, vol.9, pp.192-199, 1994.

D. Lelievre, V. P. Terrier, A. F. Delmas, and V. Aucagne, Native chemical ligation strategy to overcome side reactions during Fmoc-based synthesis of C-terminal cysteine-containing peptides, Org Lett, vol.18, pp.920-923, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01407492

M. Luconi, V. Carloni, F. Marra, P. Ferruzzi, G. Forti et al., Increased phosphorylation of AKAP by inhibition of phosphatidylinositol 3-kinase enhances human sperm motility through tail recruitment of protein kinase A, J Cell Sci, vol.117, pp.1235-1246, 2004.

M. Luconi, F. Marra, L. Gandini, E. Filimberti, A. Lenzi et al., Phosphatidylinositol 3-kinase inhibition enhances human sperm motility, Hum Reprod, vol.16, pp.1931-1937, 2001.

M. Luconi, I. Porazzi, P. Ferruzzi, S. Marchiani, G. Forti et al., Tyrosine phosphorylation of the a kinase anchoring protein 3 (AKAP3) and soluble adenylate cyclase are involved in the increase of human sperm motility by bicarbonate, Biol Reprod, vol.72, pp.22-32, 2005.

K. Luna-ramirez, V. Quintero-hernandez, L. Vargas-jaimes, C. V. Batista, K. D. Winkel et al., Characterization of the venom from the Australian scorpion Urodacus yaschenkoi: molecular mass analysis of components, cDNA sequences and peptides with antimicrobial activity, Toxicon, vol.63, pp.44-54, 2013.

Y. Ma, Y. He, R. Zhao, Y. Wu, W. Li et al., Extreme diversity of scorpion venom peptides and proteins revealed by transcriptomic analysis: implication for proteome evolution of scorpion venom arsenal, J Proteomics, vol.75, pp.1563-1576, 2012.

F. Mende and O. Seitz, Fluorenylmethoxycarbonyl-based solid-phase synthesis of peptide alpha-thioesters, Angew Chem Int Ed Engl, vol.9, pp.1232-1240, 2011.

G. P. Miljanich, Ziconotide: neuronal calcium channel blocker for treating severe chronic pain, Curr Med Chem, vol.11, pp.3029-3040, 2004.

M. Miyashita, J. Otsuki, Y. Hanai, Y. Nakagawa, and H. Miyagawa, Characterization of peptide components in the venom of the scorpion Liocheles australasiae (Hemiscorpiidae), Toxicon, vol.50, pp.428-437, 2007.

J. Nagao, M. Miyashita, Y. Nakagawa, and H. Miyagawa, Chemical synthesis of La1 isolated from the venom of the scorpion Liocheles australasiae and determination of its disulfide bonding pattern, J Pept Sci, vol.21, pp.636-643, 2015.

R. Nosrati, M. Vollmer, L. Eamer, S. Gabriel, M. C. Zeidan et al., Rapid selection of sperm with high DNA integrity, Lab Chip, vol.14, pp.1142-1150, 2014.

S. J. Publicover and C. L. Barratt, Sperm motility: things are moving in the lab, Mol Hum Reprod, vol.17, pp.453-456, 2011.

V. Quintero-hernandez, S. Ramirez-carreto, M. T. Romero-gutierrez, L. L. Valdez-velazquez, B. Becerril et al., Transcriptome analysis of scorpion species belonging to the Vaejovis genus, PLoS One, vol.10, p.117188, 2015.

B. Ravaux, N. Garroum, E. Perez, H. Willaime, and C. Gourier, A specific flagellum beating mode for inducing fusion in mammalian fertilization and kinetics of sperm internalization, Sci Rep, vol.6, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01357925

J. M. Ribeiro, J. M. Anderson, N. C. Manoukis, Z. Meng, and I. M. Francischetti, A further insight into the sialome of the tropical bont tick, Amblyomma variegatum, BMC Genomics, vol.12, p.136, 2011.

W. E. Roudebush, Role of platelet-activating factor in reproduction: sperm function, Asian J Androl, vol.3, pp.81-85, 2001.

W. B. Schill, Increased fructolysis of kallikrein-stimulated human spermatozoa, Andrologia, vol.7, pp.105-107, 1975.

T. J. Sheldon, I. Miguel-aliaga, A. P. Gould, W. R. Taylor, and D. Conklin, A novel family of single VWC-domain proteins in invertebrates, FEBS Lett, vol.581, pp.5268-5274, 2007.

E. C. Silva, T. S. Camargos, A. Q. Maranhao, I. Silva-pereira, L. P. Silva et al., Cloning and characterization of cDNA sequences encoding for new venom peptides of the Brazilian scorpion Opisthacanthus cayaporum, Toxicon, vol.54, pp.252-261, 2009.

P. R. Somanath, S. L. Jack, and S. Vijayaraghavan, Changes in sperm glycogen synthase kinase-3 serine phosphorylation and activity accompany motility initiation and stimulation, J Androl, vol.25, pp.605-617, 2004.

S. Tardif, O. A. Madamidola, S. G. Brown, L. Frame, L. Lefievre et al., Clinically relevant enhancement of human sperm motility using compounds with reported phosphodiesterase inhibitor activity, Hum Reprod, vol.29, pp.2123-2135, 2014.

I. Tasdemir, M. Tasdemir, and S. Tavukcuoglu, Effect of pentoxifylline on immotile testicular spermatozoa, J Assist Reprod Genet, vol.15, pp.90-92, 1998.

V. P. Terrier, H. Adihou, M. Arnould, A. F. Delmas, and V. Aucagne, A straightforward method for automated Fmoc-based synthesis of bio-inspired peptide crypto-thioesters, Chem Sci, vol.7, pp.339-345, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01407765

P. Terriou, E. Hans, R. Cortvrindt, C. Avon, O. Charles et al., Papaverine as a replacement for pentoxifylline to select thawed testicular or epididymal spermatozoa before ICSI

, Gynecol Obstet Fertil, vol.43, pp.786-790, 2015.

P. Thonneau, S. Marchand, A. Tallec, M. L. Ferial, B. Ducot et al., Incidence and main causes of infertility in a resident population (1,850,000) of three French regions, Hum Reprod, vol.6, pp.811-816, 1988.

H. Tournaye, M. Van-der-linden, E. Van-den-abbeel, P. Devroey, and S. A. Van, The effect of pentoxifylline on mouse in-vitro fertilization and early embryonic development, Hum Reprod, vol.9, pp.1903-1908, 1994.

I. Vetter, J. L. Davis, L. D. Rash, R. Anangi, M. Mobli et al., Venomics: a new paradigm for natural products-based drug discovery, Amino Acids, vol.40, pp.15-28, 2011.

C. Wambergue, R. Zouari, F. Ben, M. S. Martinez, G. Devillard et al., Patients with multiple morphological abnormalities of the sperm flagella due to DNAH1 mutations have a good prognosis following intracytoplasmic sperm injection, Hum Reprod, vol.31, pp.1164-1172, 2016.

, World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen