A. G. Et-edwardsson-k, Letter: Radiation-induced single-strand breaks in DNA determined by rate of alkaline strand separation and hydroxylapatite chromatography: an alternative to velocity sedimentationI,n ternational, Journal Of Radiation Biology and Related Studiesi n Physics Chemistry and Medicine, issue.25, pp.6-493, 1974.

A. , A. Hussein-hk, . Schulpen-s, . Karbaschi-m, . Abdalla-i et al., Simplified method for the collection, storage, and comet assay analysis of DNA damage in whole blood, Free Radical Biology and Medicine, issue.3, pp.51-719, 2011.

A. Fábio, P. Santos-hélio-b, . Rizzo-elizete, . Sato-yoshimi, and . Bazzoli-nilo, Influence of water temperature on induced reproduction by hypophysation,sex steroids concentrations and final oocyte maturation of the ''curimatã-pacu''Prochilodus argenteus (Pisces: Prochilodontidae), Journal of General and Comparative Endocrinology, vol.172, pp.400-408, 2011.

A. A. Gutzkow-kb, C. Brunborg-g, and . Ar, Towards a more reliable comet assay: Optimising agarose concentration, unwinding time and electrophoresis conditions, Mutation Research, vol.724, pp.41-45, 2011.

B. K. and C. K. Kirsch-volders, Development and validation of the in vivo alkaline comet assay for detecting genomic damage in marine flatfish, Mutation Research ? Genetic Toxicology and Environemental Mutagenesis, issue.3, pp.415-167, 1998.

B. Ke and . M. Christensen-g, Effects of various metols on survival, growth, reproduction and metobolism of Daphnia magna, J. Fish. Res. Bd. Canada, vol.29, pp.1691-1700, 1972.

B. , C. , and J. Jj, Fluorometric method for rapid detection of DNA strand breaks in humanw hiteb lood cellsp roducedb y low dose of radiation Cancer Research, pp.889-1892, 1981.

B. Cwm, V. Pa, and Z. D. , Ecdysteroids in Daphnia magna: Their role in moulting and reproduction and their levels upon exposure to cadmium, Aquatic Toxicology, vol.17, issue.4, pp.339-350, 1990.

B. L. Et and C. F. , Sexual selection on male body size and assortative pairing in Gammarus pulex (Crustacea: Amphipoda): field surveys and laboratory experiments, Journal of Zoology, vol.264, issue.2, pp.135-141, 2004.

B. Pc, Internal hazards: baseline DNA damage by endogenus products of normal metabolism, Mut Res, vol.433, pp.11-36, 1999.

C. Rf and L. J. , Environmental-effects on numbers of peripheral chemoreceptors on the antennae of a grasshopper, Chemical Senses, vol.16, issue.6, pp.607-616, 1991.

C. L. Dehaan and H. Ursprung, Hormonal control of sex differentiation in invertebrates, Organogenesis. R, pp.701-740, 1965.

C. L. Geffard-o, C. A. , C. R. , Q. H. , and G. A. Dedourge-geffard, Effect of water quality and confounding factors on digestive enzyme activities in Gammarus fossarum, Environ Sci Pollut Res Int, vol.20, issue.12, pp.9044-9056, 2013.

C. L. Geffard-o, C. A. , C. R. Jaffa, . Gaillet-v, . O. Debourge-geffard et al., Influence of molting and starvation on digestive enzyme activities and energy storage in Gammarus fossarum, PloS One, vol.9, p.96393, 2014.

C. S. and K. A. Statzner, Biomonitoring through biological traits of benthic macroinvertebrates : perspectives for a general tool in stream management, Archive für Hydrobiology, vol.142, issue.4, pp.415-432, 1998.

C. Miao-lin and . Hu, Use of whole blood directly for single-cell gel electrophoresis (comet) assay in vivo and white blood cells for in vitro assay, Genetic Toxicology and Environmental Mutagenesis. Mutation Research, vol.564, pp.75-82, 2004.

C. É. , F. L. Chinnery, F. E. Et-williams, and J. A. , Amphipodes Paris Photoperiod and temperature regulation of diapause egg production in Acartia bifilosa from Southampton Water, Marine Ecology Progress Series, vol.263, pp.149-157, 1970.

C. Ld, . Houk-vs, and . Hughes-tj, Genotoxicity of industrial wastes and effluents, Mutation Research -Reviews in Mutation Research, vol.410, issue.3, pp.237-243, 1998.

C. A. and F. Ve, Consequences of a short pulse of pesticide exposure for survival and reproduction of Gammaurd pulex, Aquatic Toxicology, vol.67, issue.3, pp.287-299, 2004.

C. A. Dobson-vl, K. G. Dusisnska-m, and . Stetina-r, The comet assay: what can it really tell us?, Mutation Research, vol.375, pp.183-193, 1997.

C. N. Sirard and . Bailey-j-l, Premature capacitation of bovine spermatozoa is initiated by cryopreservation, J. Androl, vol.18, pp.461-468, 1997.

D. Palais-f, . S. Biagianti-risbourg, . Geffard-o, and . Geffard-a, Effects of metals on feeding rate and digestive enzymes in Gammarus fossarum: An in situ experiment, Chemosphere, vol.77, issue.11, pp.1569-1576, 2009.

D. A. Pesonen-m and . Monod-g, Alkaline comet assay in rainbow trout hepatocytes, Toxicol in vitro, vol.11, pp.71-79, 1997.

D. A. Pesonen-m and . Monod-g, Alkaline comet assay in rainbow trout hepatocytes, Toxicol. In Vitro, vol.11, pp.71-79, 1997.

D. A. Flammarion-p, G. J. Bernardon-v, and . Monod-g, Monitoring of the chemical pollution of the river Rhône through measurement of DNA damage and cytochrome P4501ainduction in chub (Leuciscus cephalus), Mar Environ Res, vol.46, pp.1-5, 1998.

D. A. Fiat-l, . Gillet-c, and . Bony-s, Reproduction impairment following paternal genotoxin exposure in brown trout(Salmo trutta) and Arctic charr (Salvelinus alpinus), Journal of aquatic ecotoxicology, vol.101, issue.2, pp.405-411, 2011.

D. A. Bony-s, Genotoxicity of contaminants: Comet assay Encyclopedia of Aquatic Ecotoxicology, Springer Science + Business Media Dordrecht, pp.559-568, 2013.

D. Dr, P. A. Dixon-m, and A. Jha, Marine invertebrate ecogenotoxicology : A methodological overview, Mutagenesis, vol.17, issue.6, pp.495-507, 2002.

D. S. Statzner-b and . Bournard-m, Species traits for future biomonitoring across ecoregions: patterns along a human-impacted river, Freshwater Biology, vol.42, pp.737-758, 1999.

E. and D. Cooke-m-s, Factors contributing to the outcome of oxidative damage to nucleic acids, Bioessays, vol.26, issue.5, pp.533-538, 2004.

F. V. , G. Charmantier, G. A. Mons-r, R. P. , C. M. et al., Physiological and behavioural responses of Gammarus pulex (Crustacea: Amphipoda) exposed to cadmium, Aquatic Toxicology, vol.86, issue.3, pp.413-425, 2008.

F. V. Tixier-g, . Guérold-f, . De-crespin-d-b, and . Dangles-o, Quantification of diet variability in a stream amphipod: implications for ecosystem functioning, Fundamental and Applied LimnologyArchiv für Hydrobiologie, vol.170, issue.4, pp.303-313, 2008.

F. and S. Fairfull, The effects of rapid cooling ( cold shock) of Ram Semen, photoperiod, and egg yolk in diluents on the survival of spermatozoa before and after freezing, Cryobiology, vol.23, pp.518-524, 1986.

G. , C. , and A. Blaise-c, The dual nature of metallothioneins in the metabolism of heavy metals and reactive oxygen species in aquatic organisms: implications of use as a biomarkerof heavy-metal effects in field investigations, Biochemistry Insights, vol.1, pp.23-33, 2008.

G. J. Ferard-jf and . Vindimian-e, Essais biologiques pour /'estimation de la toxicité chronique des effluents. Numéro spécial. rapport final, 1992.

G. A. Geffard-o, A. J. His-e, and . C. Amiard-triquet, Bioaccumulation of Metals in Sediment Elutriates and Their Effects on Growth, Condition Index, and Metallothionein Contents in Oyster Larvae, Archives of Environmental Contamination and Toxicology, vol.249, issue.1, pp.57-65, 2007.
DOI : 10.1007/s00244-006-0046-y

G. O. Xuereb-b, C. A. , G. A. Biagianti-s, A. K. Noël-c, G. J. et al., Ovarian cycle and embryonic development in Gammarus fossarum: Application for Reproductive Toxicity Assessment, Environmental Toxicology and Chemistry, issue.10, pp.29-2249, 2010.

G. A. Fenske-m, T. C. Verslycke, and . Janssen-c, Development of a quantitative enzyme-linked immunosorbent assay for vitellin in the mysid Neomysis integer (Crustacea: Mysidacea), Comparative Biochemistry and Physiology, vol.142, pp.43-49, 2005.

G. M. Fahy, The Relevance of Cryoprotectant, Cryobiology. RYOBIOLOGY, vol.23, p.13, 1986.

H. B. Kelley-r-n and A. , Cryopreservation of zebrafish spermatozoa using methanol, Canadian Journal of Zoology, vol.60, pp.1867-1870, 1982.

H. Es and . Kim-gh, Biomarkers for oxidative stress status of DNA, lipids, and proteins in vitro and in vivo cancer research, Toxicology, vol.229, pp.1-10, 2007.

H. Rv, Review of the reproductive biology of amphipods and their endocrine regulation: identification of mechanistic pathways for reproductive toxicants, Environ Toxicol Chem, vol.30, issue.12, pp.2647-2657, 2011.

J. An, Ecotoxicological applications and significance of the comet assay, Mutagenesis, vol.23, issue.3, pp.207-221, 2008.

J. E. Parks and D. V. Lynch, Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes, Cryobiology, vol.29, issue.2, pp.255-266, 1992.
DOI : 10.1016/0011-2240(92)90024-V

K. and S. S. Pinkste, Freshwater Gammarus species from Europe, north Africa and adjacent regions of Asia (Crustacea-Amphipoda), part I. Gammarus pulex-group and related species, 1977.

K. and A. Lambert-c-c, Ultrastructure of sperm, spermiogenesis, and sperm-egg interactions in selected invertebrates and lower-vertebrates which use external fertilization, Journal of Electron Microscopy Technique, vol.16, pp.115-154, 1990.

K. , Y. Kienle-c, and G. A. , Gammarus spp. in Aquatic Ecotoxicology and Water Quality Assessment: Toward Integrated Multilevel Tests. Rev of Environ Contam Toxicol, pp.1-76, 2010.

L. E. Geffard-o, . Bony-s, and . Devaux-a, Genotoxicity assessment in the amphipod Gammarus fossarum by use of the alkaline Comet assay, Mutation Research ? Gen Toxicol Environ Mutag, vol.700, issue.12, pp.32-38, 2010.

L. E. , D. A. , J. G. Mons-r, Q. H. Gardette-m, G. J. Bony-s et al., DNA damage in Gammarus fossarum sperm as a biomarker of genotoxic pressure: intrinsic variability and reference level, Sci Total Environ, vol.409, issue.17, pp.3230-3236, 2011.

L. E. , D. A. Mons-r, G. J. Bony-s, and G. A. Geffard-o, DNA damage in caged Gammarus fossarum amphipods: A tool for freshwater genotoxicity assessment, Environmental Pollut, issue.6, pp.159-1682, 2011.

L. E. Geffard-o, B. S. Goyet-d, and . Devaux-a, Linking genotoxic responses in Gammarus fossarum germ cells with reproduction impairment, using the Comet assay, Environ Res, vol.111, issue.5, pp.626-634, 2011.

L. V. , J. D. , K. Hr, . Schirling-m, . Triebskorn-r et al., Population structure and dynamics of Gammarus fossarum (Amphipoda) upstream and downstream from effluents of sewage treatment plants, Arch Environ Contam Toxicol, issue.3, pp.50370-383, 2006.

L. Sa, . Knaapen-am, . Brauers-kj, . Van-berlo-d, . Van-schooten-fj et al., Development and validation of a modified comet assay to phenotypically assess nucleotide excision repair, Mutagenesis, vol.21, pp.153-158, 2006.

L. A. and J. C. Poulter, Development of a sub-lethal pollution bioassay using the estuarine amphipod Gammarus duebeni, Water Research, vol.32, issue.3, pp.569-578, 1998.

L. J. , R. F. , and C. S. Bollache-l, sperm depletion, male mating behaviour and reproductive " time-out " in gammarus pulex ( Crustacea, Amphipoda), Animal behaviour, vol.77, issue.1, pp.49-54, 2009.

L. Roux and A. , Description d'organes mandibulaires nouveaux chez les crustacés décapodes, C R Acad Sci Paris Ser D, vol.266, pp.1414-1417, 1968.

L. C. and F. At, Infertility in male aquatic invertebrates: A review, Journal of aquatic toxicology, vol.120121, pp.79-89, 2012.

M. Tt and . Mackereth-tc, Notes on Gammarus pulex in the English Lake District, Hydrobiol, vol.9, pp.1-12, 1957.

M. C. , D. J. Bigsby-e, M. W. Elwood-rw, . Gibbins-cn, and . Kelly-dw, The validity of the Gammarus : Asellus ratio as an index of organic pollution: abiotic and biotic influences, WATER RESEARCH, vol.36, issue.1, pp.75-84, 2002.

M. L. Et-naylor-c, Preliminary observations on the ecological relevance of the Gammarus scope for growth'assay: effect of zinc on reproduction, Functional Ecology, pp.393-397, 1990.

M. L. Clayton-s-a, . Wood-r-m, and . Maloughlin-n, Evaluation of the Gammarus pulex in situ feeding assay as abiomonitor of water quality: Robustness, responsiveness, and relevance, Environ Toxicol Chem, vol.21, issue.2, pp.361-368, 2002.

M. E. Bechmann-r-k, T. S. , A. E. Bjørnstad-a, L. Baussant-t, and . Floch-s, The neutral red lysosomal retention assay and Comet assay on haemolymph cells from mussels (Mytilus edulis) and fish (Symphodus melops) exposed to styrene, Aquatic Toxicology, vol.10, issue.3, pp.75-191, 2005.

M. T. Fritsch-e-f and S. J. , Molecular cloning: a laboratory manual, ACTA Biothechnol, vol.5, issue.1, p.104, 1982.

M. Tuberty-sr, . Mckenney, . Cl, . J. Blasco, . Sarasquete-c et al., The use of bioaccumulation, biomarkers and histopathology diseases in shape Procambarus clarkii to establish bioavailability of Cd and Zn after a mining spill, Environmental Monitoring and Assessment, vol.116, pp.1-3169, 2006.

M. R. Martoja-pierson, Initiation aux techniques de l'histologie animale, p.345, 1967.

M. , J. Degraeve-gm, and . Linton-t-k, Sediment quality guidelines and assessment: overview and research needs, Environmental Science and Policy, vol.3, pp.133-144, 2000.

M. O. Speit-g, Detection of crosslinks with the comet assay in relationship togenotoxicity and cytotoxicity, Environmental and Molecular Mutagenesis, vol.33, issue.2, pp.167-172, 1999.

M. Cl and C. Jk, DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring, Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, vol.399, issue.2, pp.135-147, 1998.

M. T. Dimitrov-s-l and . Houde-d, UV-Laser Crosslinking of Proteins to DNA, Methods, vol.11, issue.2, pp.225-234, 1997.

M. Rm, R. Dm, and . Shutes-rbe, A statistical approach for the assessment of the toxic influences on Gammarus pulex (Amphipoda) and Asellus aquaticus (Isopoda) exposed to urban aquatic discharges, Water research, vol.30, pp.1237-1243, 1996.

O. T. Watanabe-t and . Wakabayashi-k, Mutagens in surface waters, Mutat Res, vol.567, issue.2-3, pp.109-149, 2004.

O. P. , L. P. Banáth, and . Durand-r-e, Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells using the "Comet assay, Radiat Res, vol.122, issue.1, pp.86-94, 1990.

P. S. And and W. H. , Detection of DNA-crosslinking agents with the alkaline comet assay, Environmental and Molecular Mutagenesis, vol.27, issue.3, pp.196-201, 1996.

P. E. Batelier-f, . Duchamp-g, L. Furtoss-v, K. Y. Vern, and . Vidament-m, Freezing stallion semen in INRA96-based extender improves fertility rates in comparison with INRA82, Dairy Sci Technol, vol.88, pp.257-265, 2008.

P. M. Humpesch-u, Intra?and inter?specific variations in egg survival and brood development time for Austrian populations of Gammarus fossarum and G. roeseli (Crustacea: Amphipoda), Freshwater Biology, vol.23, issue.3, pp.441-455, 1990.

Q. P. and J. White-i-g, Phospholipid and cholesterol content of epididymal and ejaculated ram spermatozoa and seminal plasma in relation to cold shock, J.Biol.Sci, vol.20, pp.1205-1215, 1967.

R. Jf, . Itaya-p-w, and . Fitzgerald-m-a, A thin section and freeze-fracture study on membrane specialization in spermatozoa of the isopod, armadillidium vulgare, Journal of ultrastructure rescherch, vol.67, issue.2, pp.180-193, 1979.

R. Mr, M. Da, and . Fingerman-m, Endocrinedisruption in crustaceans due to pollutants : A review, Comparative Biochemistry and Physiology, pp.661-671, 2007.

R. , A. Goff-hd, . Senaratne-v, . Dalgliesh-d-g, and . Flores-a, Gelation of commercial fractions of ?-lactoglobulin and ?-lactalbumin, International Dairy Journal, vol.7, pp.79-85, 1997.

R. B. Johanson, Estimation of single strand breaks in single mammalian cells, DNA Repair Mecanisms, Academic press, pp.465-468, 1978.

S. M. , J. D. Ladewig-v, L. Ku, K. Nagel-r, and . Hr, Bisphenol A in artificial indoor streams: II. Stress response and gonad histology in Gammarus fossarum (Amphipoda), Ecotoxicology, vol.15, issue.2, pp.143-156, 2006.

S. Eh, Amphipoda, Microscopic Anatomy of Invertebrates: john wiley and son, pp.443-528, 1992.

S. and D. C. Cantor-c-r, Separation of yeast chromosome-sized D NA by pulsed field gradient gel electrophoresis Cell, pp.67-75, 1984.

S. E. Rajapakse-n and . Kortenkamp-a, Something from "nothing"--eight weak estrogenic chemicals combined at concentrations below NOECs produce significant mixture effects, 2002.

S. , P. Macoy-m-t, . Tice-r-r, and . Schneider-e-l, A simple technique for quantitation of low levels of DNA damage in individual cells, Experimental Cell Res, vol.175, issue.1, pp.184-191, 1988.

S. , P. Danner-d-b, . Tice-r-r, J. D. Pearson, M. H. Brant-l-j et al., Basal DNA damage in individual human lymphocytes with age, Mutat Res, vol.256, issue.1, pp.1-6, 1991.

S. S. Cossu-leguille, Effects of sublethal copper exposure on two gammarid species: which is the best competitor?, Ecotoxicology, vol.20, issue.1, pp.264-273, 2011.

S. and S. C. Cossu-leguille, Seasonal variability of antioxidant biomarkers and energy Reserves in the freshwater gammarid Gammarus roeseli, Chemosphere, vol.83, issue.4, pp.538-544, 2011.

S. G. Birkey-m and . Hulett-fm, Three two component signal transduction systems interact for pho regulation in Bacillus subtilis, Mol Microbiol, vol.19, issue.5, pp.941-848, 1996.

S. Dh and . Steele-vj, Biology of Gammarellus angulosus (Crustacea, Amphipoda) in the northwestern Atlantic, Journal of the Fisheries Research Board of Canada, vol.29, issue.9, pp.1337-1340, 1972.

T. H. Bournaud-m and R. P. Usseglio-polaterra, Invertebrés d'eau douce : Systematiques, bilgie, écologie, CNRS édition, p.588, 2000.

T. F. Verneau-o, C. F. Santalla-f, and R. F. , The influence of intensity of infection by a trematode parasite on the reproductive biology of Gammarus insensibilis (Amphipoda), International journal for parasitology, vol.26, issue.11, pp.1205-1209, 1996.

T. R. Agurell-r-e, A. D. Burlinson-b, K. H. Hartmann-a, S. Miyanae-y, . Rojas-e et al., Single cell gel/Comet assay: Guidelines for in vitro and invivo genetic toxicology testing, Environmental and Molecular Mutagenesis, vol.35, pp.206-221, 2000.

T. S. Bendena-wg, The regulation of juvenile hormone production in arthropods - Functional and evolutionary perspectives, Annals of The New York Academy of Sciences, vol.897, pp.300-310, 1999.

V. Sonntag and C. , New Aspects in the Free-Radical Chemistry of Pyrimidine Nucleobases, Free Radical Research Communications, vol.38, issue.4-6, pp.217-224, 1987.
DOI : 10.3109/10715768709065286

W. R. White-i, The susceptibility of spermatozoa to temperature shock, J. Endocrin, vol.19, pp.211-220, 1959.

W. , R. Mcglynn-a-p, . Mcnulty-h, . O-'reilly-s-l, . J. Mckelvey-martin-v et al., Global DNA and p53 region-specific hypomethylation in human colonic cells is induced by folate depletion and reversed by folate supplementation, J Nutr, issue.11, pp.136-2748, 2006.

W. P. Lewandowska-r, . Brzuzan-p, . Ziomek-e, and . Bardega-ry, The level of DNA damage and the frequency of micronuclei in haemolymph of freshwater mussels Anodonta woodiana exposed to Benzo[A]Pyrene, Acta Toxicologica, vol.12, issue.1, pp.41-45, 2004.

X. B. Noury-p, G. J. Felten-v, and . Geffard-o, Cholinesterase activity in Gammarus pulex (Crutacea Amphipoda): Characterization and effects of chlorpyrifos, Toxicolgy, vol.236, issue.3, pp.178-189, 2007.

X. B. , C. A. Mons-r, and G. J. Geffard-o, Acetylcholinesterase activity in Gammarus fossarum (Crustacea Amphipoda) Intrinsic variability, reference levels, and a reliable tool for field surveys, Aquat Toxicol, vol.93, issue.4, pp.225-233, 2009.

X. B. Lefevre-e and G. J. Geffar-o, Acetylcholinesterase activity in Gammarus fossarum (Crustacea Amphipoda): linking AChE inhibition and behavioural alteration, Aquat Toxicol, vol.94, issue.2, pp.114-122, 2009.

X. B. Bezin-l, C. A. Budzinski-h, A. S. Tutundjian-r, and G. J. Geffard-o, Vitellogenin-like gene expression in freshwater amphipod Gammarus fossarum (Koch, 1835): functional characterization in females and potential for use as an endocrine disruption biomarker in males, Ecotoxicology, vol.20, issue.6, pp.1286-1299, 2011.

Y. H. Carmichael-c and T. T. Zoltan-m-v, Development of simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio, Theriogenology, pp.68-128, 2007.