. Décret, établissant les règes particulières de prévention des risques cancérogènes, mutagènes ou toxiques pour la reproduction et modifiant le code du travail. 2. Sous-section 6: Règles particulières de prévention à prendre contre les risques d'exposition aux agents cancérogènes, mutagènes ou toxiques pour la reproduction Code du travail, pp.231-56, 2001.

F. Plénat, Les cons??quences mol??culaires de la fixation et de l???inclusion: exemple des acides nucl??iques et des prot??ines, Annales de Pathologie, vol.26, issue.1, pp.8-21, 2006.
DOI : 10.1016/S0242-6498(06)70655-1

L. Remontet, Cancer incidence and mortality in France over the period 1978-2000. Revue d'épidémiologie et de santé publique, pp.3-30, 2003.
URL : https://hal.archives-ouvertes.fr/hal-00427472

A. Belot, Cancer incidence and mortality in France over the period 1980-2005. Revue d'épidémiologie et de santé publique, pp.159-75, 2008.
URL : https://hal.archives-ouvertes.fr/hal-00538673

Y. Martinet, Le cancer bronchique vu par un pneumologue Bulletin de la Division Française de l'AIP, pp.5-7, 2005.

R. S. Herbst, J. V. Heymach, and S. M. Lippman, Lung Cancer, New England Journal of Medicine, vol.359, issue.13, pp.359-1367, 2008.
DOI : 10.1056/NEJMra0802714

D. Travis and W. B. , Pathology and genetics of tumours of the lung, pleura, thymus and heart -Page 305, Bronchopulmonary neuroendocrine tumors. Cancer, vol.113, issue.1, pp.5-21, 2004.

M. C. Copin, Apport de l'immunohistochimie au diagnostic d'une tumeur pulmonaire. Bulletin de la Division Française de l'AIP, pp.47-53, 2005.

P. G. Chu and L. M. Weiss, Keratin expression in human tissues and neoplasms, Histopathology, vol.72, issue.5, pp.403-442, 2002.
DOI : 10.1007/s004280050080

G. Buccheri and D. Ferrigno, Lung tumor markers of cytokeratin origin: an overview, Lung Cancer, vol.34, issue.2, pp.65-74, 2001.
DOI : 10.1016/S0169-5002(01)00347-6

J. Viac, Reactivity Pattern of a Monoclonal Antikeratin Antibody (KL1), Journal of Investigative Dermatology, vol.81, issue.4, pp.351-355, 1983.
DOI : 10.1111/1523-1747.ep12519941

R. Camilo, Expression of p63, keratin 5/6, keratin 7, and surfactant-A in non???small cell lung carcinomas, Human Pathology, vol.37, issue.5, pp.542-548, 2006.
DOI : 10.1016/j.humpath.2005.12.019

Y. C. Su, Y. C. Hsu, and C. Y. Chai, Role Of TTF-1, CK20, And CK7 Immunohistochemistry for Diagnosis of Primary and Secondary Lung Adenocarcinoma, The Kaohsiung Journal of Medical Sciences, vol.22, issue.1, pp.14-23, 2006.
DOI : 10.1016/S1607-551X(09)70214-1

J. Marson and V. , Expression of TTF-1 and cytokeratins in primary and secondary epithelial lung tumours: correlation with histological type and grade, Histopathology, vol.29, issue.2, pp.125-159, 2004.
DOI : 10.1038/modpathol.3880044

J. Jagirdar, Application of immunohistochemistry to the diagnosis of primary and metastatic carcinoma to the lung, Arch Pathol Lab Med, vol.132, issue.3, pp.384-96, 2008.

Y. Yatabe, T. Mitsudomi, and T. Takahashi, TTF-1 Expression in Pulmonary Adenocarcinomas, The American Journal of Surgical Pathology, vol.26, issue.6, pp.767-73, 2002.
DOI : 10.1097/00000478-200206000-00010

S. Lantuejoul, Neural Cell Adhesion Molecules (NCAM) and NCAM-PSA Expression in Neuroendocrine Lung Tumors, The American Journal of Surgical Pathology, vol.22, issue.10, pp.22-1267, 1998.
DOI : 10.1097/00000478-199810000-00012

M. C. Howe, Neuroendocrine differentiation in non-small cell lung cancer and its relation to prognosis and therapy, Histopathology, vol.107, issue.2, pp.195-201, 2005.
DOI : 10.1002/(SICI)1096-9896(199705)182:1<13::AID-PATH804>3.3.CO;2-Q

N. Louis, D. , W. K. Cavenee, and H. Ohgaki, WHO classification of tumours of the central nervous system, p.309, 2007.

J. M. Bonnin and L. J. Rubinstein, Immunohistochemistry of central nervous system tumors, Journal of Neurosurgery, vol.60, issue.6, pp.1121-1154, 1984.
DOI : 10.3171/jns.1984.60.6.1121

L. F. Eng, R. S. Ghirnikar, and Y. L. Lee, Glial fibrillary acidic protein: GFAP-thirty-one years, Neurochem Res, vol.25, pp.9-10, 1969.

H. K. Wolf, NeuN: a useful neuronal marker for diagnostic histopathology., Journal of Histochemistry & Cytochemistry, vol.44, issue.10, pp.1167-71, 1996.
DOI : 10.1177/44.10.8813082

T. Gotow, Neurofilaments in health and disease. Medical electron microscopy : official journal of the Clinical Electron Microscopy Society of Japan, pp.173-99, 2000.

L. Taupenot, K. L. Harper, and D. T. Connor, The Chromogranin???Secretogranin Family, New England Journal of Medicine, vol.348, issue.12, pp.348-1134, 2003.
DOI : 10.1056/NEJMra021405

H. Takei, New immunohistochemical markers in the evaluation of central nervous system tumors: a review of 7 selected adult and pediatric brain tumors Molecular testing in solid tumors: an overview, Arch Pathol Lab Med Arch Pathol Lab Med, vol.131, issue.22, pp.234-275, 2007.

M. Buyse, Validation and Clinical Utility of a 70-Gene Prognostic Signature for Women With Node-Negative Breast Cancer, JNCI Journal of the National Cancer Institute, vol.98, issue.17, pp.98-1183, 2006.
DOI : 10.1093/jnci/djj329

T. De-la-motte-rouge, [Clinical and molecular predictors of response to EGFR tyrosine kinase inhibitors in non-small cell lung cancer] Annales de pathologie Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib, N Engl J Med, vol.27, issue.3221, pp.353-63, 2004.

K. Aldape, P. C. Burger, and A. Perry, Clinicopathologic aspects of 1p/19q loss and the diagnosis of oligodendroglioma, Arch Pathol Lab Med, vol.131, issue.2, pp.242-51, 2007.

J. A. Ramos-vara, Technical Aspects of Immunohistochemistry, Veterinary Pathology, vol.98, issue.4, pp.405-431, 2005.
DOI : 10.1146/annurev.bi.50.070181.003301

S. M. Hewitt, Tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, paraffin-embedded tissue, Arch Pathol Lab Med, issue.12, pp.132-1929, 2008.

F. Plénat, [Formaldehyde fixation in the third millennium], Annales de pathologie, vol.21, issue.37, pp.29-47, 2001.

J. A. Kiernan, Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: What they are and what they do Microscopy Today, pp.8-12, 2000.

J. D. Mcghee and P. H. , Formaldehyde as a probe of DNA structure. I. Reaction with exocyclic amino groups of DNA bases, Biochemistry, vol.14, issue.6, pp.14-1281, 1975.
DOI : 10.1021/bi00677a029

J. D. Mcghee and P. H. , Formaldehyde as a probe of DNA structure. II. Reaction with endocyclic imino groups of DNA bases, Biochemistry, vol.14, issue.6, pp.14-1297, 1975.
DOI : 10.1021/bi00677a030

N. Masuda, Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples, Nucleic Acids Research, vol.27, issue.22, pp.27-4436, 1999.
DOI : 10.1093/nar/27.22.4436

M. Srinivasan, D. Sedmak, and S. Jewell, Effect of Fixatives and Tissue Processing on the Content and Integrity of Nucleic Acids, The American Journal of Pathology, vol.161, issue.6, pp.161-1961, 2002.
DOI : 10.1016/S0002-9440(10)64472-0

L. A. Loeb, Apurinic sites as common intermediates in mutagenesis Basic Life Sci DNA damage caused by common cytological fixatives, Mutat Res, vol.38, issue.4011 2, pp.341-348, 1986.

D. Brutlag, C. Schlehuber, and J. Bonner, Properties of formaldehyde-treated nucleohistone, Biochemistry, vol.8, issue.8, pp.3214-3222, 1969.
DOI : 10.1021/bi00836a013

R. D. Start, Reassessment of the rate of fixative diffusion., Journal of Clinical Pathology, vol.45, issue.12, pp.1120-1121, 1992.
DOI : 10.1136/jcp.45.12.1120

M. Werner, Effect of formalin tissue fixation and processing on immunohistochemistry Formaldehyde fixation, Am J Surg Pathol J Histochem Cytochem, vol.24, issue.78, pp.33-845, 1985.

K. G. Helander, Kinetic Studies of Formaldehyde Binding in Tissue, Biotechnic & Histochemistry, vol.21, issue.3, pp.177-186, 1994.
DOI : 10.1177/33.8.3894502

J. A. Ramos-vara, M. A. Miller, and G. C. Johnson, Usefulness of thyroid transcription factor-1

J. T. Mason and T. J. Leary, Effects of formaldehyde fixation on protein secondary structure: a calorimetric and infrared spectroscopic investigation., Journal of Histochemistry & Cytochemistry, vol.39, issue.2, pp.225-234, 1991.
DOI : 10.1177/39.2.1987266

L. Antunes, Possible role of tissue shrinkage in high-temperature antigen retrieval, Histopathology, vol.48, issue.4, pp.471-474, 2006.
DOI : 10.1002/(SICI)1096-9896(199706)182:2<233::AID-PATH827>3.3.CO;2-P

S. R. Shi, R. J. Cote, and C. R. Taylor, Antigen Retrieval Techniques, Journal of Histochemistry & Cytochemistry, vol.48, issue.8, pp.49-931, 2001.
DOI : 10.1177/26.7.211157

V. K. Rait, T. J. Leary, and J. T. Mason, Modeling formalin fixation and antigen retrieval with bovine pancreatic ribonuclease A: I???Structural and functional alterations, Laboratory Investigation, vol.84, issue.3, pp.292-301, 2004.
DOI : 10.1038/labinvest.3700045

V. K. Rait, Modeling formalin fixation and antigen retrieval with bovine pancreatic RNase A II. Interrelationship of cross-linking, immunoreactivity, and heat treatment, Laboratory Investigation, vol.84, issue.3, pp.300-306, 2004.
DOI : 10.1038/labinvest.3700041

T. E. Godfrey, Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5' nuclease quantitative reverse transcription-polymerase chain reaction. The Journal of molecular diagnostics : JMD, pp.84-91, 2000.

U. Lehmann and H. Kreipe, Real-Time PCR Analysis of DNA and RNA Extracted from Formalin-Fixed and Paraffin-Embedded Biopsies, Methods, vol.25, issue.4, pp.409-427, 2001.
DOI : 10.1006/meth.2001.1263

M. Cronin, Measurement of Gene Expression in Archival Paraffin-Embedded Tissues, The American Journal of Pathology, vol.164, issue.1, pp.35-42, 2004.
DOI : 10.1016/S0002-9440(10)63093-3

R. Coura, An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue, Journal of Clinical Pathology, vol.58, issue.8, pp.58-894, 2005.
DOI : 10.1136/jcp.2004.021352

C. Williams, A High Frequency of Sequence Alterations Is Due to Formalin Fixation of Archival Specimens, The American Journal of Pathology, vol.155, issue.5, pp.1467-71, 1999.
DOI : 10.1016/S0002-9440(10)65461-2

K. A. Eckert and T. A. Kunkel, DNA polymerase fidelity and the polymerase chain reaction., Genome Research, vol.1, issue.1, pp.17-24, 1991.
DOI : 10.1101/gr.1.1.17

T. Hultman, Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA, BioTechniques, vol.10, issue.1, pp.84-93, 1991.

S. M. Farragher, RNA expression analysis from formalin fixed paraffin embedded tissues, Histochemistry and Cell Biology, vol.71, issue.3, pp.435-480, 2008.
DOI : 10.1007/s00418-008-0479-7

J. D. Mcghee, P. H. Hippel, and F. , Formaldehyde as a probe of DNA structure. 3. Equilibrium denaturation of DNA and synthetic polynucleotides Effects of processing delay, formalin fixation, and immunohistochemistry on RNA Recovery From Formalin-fixed Paraffin-embedded Tissue Sections, Biochemistry Diagn Mol Pathol, vol.16, issue.171, pp.3267-76, 1977.

K. Specht, Quantitative Gene Expression Analysis in Microdissected Archival Formalin-Fixed and Paraffin-Embedded Tumor Tissue, The American Journal of Pathology, vol.158, issue.2, pp.419-448, 2001.
DOI : 10.1016/S0002-9440(10)63985-5

S. D. Finkelstein, Cold-temperature plastic resin embedding of liver for DNA-and RNA-based genotyping. The Journal of molecular diagnostics : JMD, pp.17-22, 1999.

D. Shibata, W. J. Martin, and N. Arnheim, Analysis of DNA sequences in forty-year-old paraffin-embedded thintissue sections: a bridge between molecular biology and classical histology, Cancer Res, vol.70, issue.16, pp.48-4564, 1988.

A. E. Kingsbury, Tissue pH as an indicator of mRNA preservation in human post-mortem brain, Molecular Brain Research, vol.28, issue.2, pp.311-319, 1995.
DOI : 10.1016/0169-328X(94)00219-5

L. Dubeau, Southern blot analysis of DNA extracted from formalin-fixed pathology specimens, Cancer Res, vol.46, issue.6, pp.2964-2973, 1986.

A. M. Shedlock, Enhanced DNA extraction and PCR amplification of mitochondrial genes from formalinfixed museum specimens Fundamental study on the mechanism of DNA degradation in tissues fixed in formaldehyde, BioTechniques J Clin Pathol, vol.22, issue.3989, pp.394-400, 1990.

R. D. Foss, Effects of Fixative and Fixation Time on the Extraction and Polymerase Chain Reaction Amplification of RNA from Paraffin-Embedded Tissue, Diagnostic Molecular Pathology, vol.3, issue.3, pp.148-55, 1994.
DOI : 10.1097/00019606-199409000-00003

A. Ribeiro-silva, H. Zhang, S. S. Jeffrey, and M. , RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies Global gene expression profiling of formalin-fixed paraffin-embedded tumor samples: a comparison to snap-frozen material using oligonucleotide microarrays, BMC Mol Biol Virchows Arch, issue.6, pp.450-699, 2007.

I. National-du-cancer and I. , Les tumorothèques Hospitalières : Recommandations à l'usage des cliniciens et des chercheurs, 2006.

A. , C. Yamashita, and S. , Gestion d'une structure ACP: recommandations et reglementations 2ème version-Août Heat-induced antigen retrieval: mechanisms and application to histochemistry, Progress in histochemistry and cytochemistry, pp.41-141, 2007.

H. Autorité-de-santé, H. Beltramo, and P. , Recommandations de Bonne Pratique: Cryopréservation de tissus, cellules et liquides biologiques issus du soin Septembre Utilisation de l'azote liquide en toute sécurité La gazette de la transfusion, pp.37-42, 1997.

S. S. Jeffrey, P. E. Lønning, and B. E. Hillner, Genomics-based prognosis and therapeutic prediction in breast cancer, Journal of the National Comprehensive Cancer Network : JNCCN, vol.3, issue.3, pp.291-300, 2005.

G. Stanta, A Novel Fixative Improves Opportunities of Nucleic Acids and Proteomic Analysis in Human Archive??s Tissues, Diagnostic Molecular Pathology, vol.15, issue.2, pp.115-138, 2006.
DOI : 10.1097/00019606-200606000-00009

S. S. Wang, Cervical Tissue Collection Methods for RNA Preservation: Comparison of Snap-frozen, Ethanol-fixed, and RNAlater-fixation, Diagnostic Molecular Pathology, vol.15, issue.3, pp.144-152, 2006.
DOI : 10.1097/01.pdm.0000213460.53021.cd

J. W. Gillespie, Evaluation of Non-Formalin Tissue Fixation for Molecular Profiling Studies, The American Journal of Pathology, vol.160, issue.2, pp.449-57, 2002.
DOI : 10.1016/S0002-9440(10)64864-X

M. L. Lacroix-triki, L. Cuider, B. Decha, A. Voigt, J. J. Rochaix et al., RCL2 a new non toxic fixative allowing extraction of high quality DNA, mRNA and protein from paraffin embedded tissues

C. Delfour, RCL2, a new fixative, preserves morphology and nucleic acid integrity in paraffin-embedded breast carcinoma and microdissected breast tumor cells. The Journal of molecular diagnostics : JMD, pp.157-69, 2006.

P. G. Chu and L. M. Weiss, Expression of Cytokeratin 5/6 in Epithelial Neoplasms: An Immunohistochemical Study of 509 Cases, Modern Pathology, vol.15, issue.1, pp.6-10, 2002.
DOI : 10.1038/modpathol.3880483